van der Noordaa J, van Haagen A, Walboomers J M, van Someren H
J Virol. 1972 Jul;10(1):67-72. doi: 10.1128/JVI.10.1.67-72.1972.
Hybrids between mouse cells and simian virus 40 (SV40)-transformed rat cells were made, and their properties and chromosome constitution were investigated over many generations. Their hybrid nature was confirmed by enzyme studies. During a period of 1 year a loss of 10 to 20% of the total number of chromosomes was observed. The SV40 tumor antigen was present and remained present in the hybrids. The parental and hybrid cells were studied for agglutination with concanavalin A, for growth in soft agar, and for serum requirement. These growth and surface characteristics of the transformed cells appeared in the hybrids.
制备了小鼠细胞与猿猴病毒40（SV40）转化的大鼠细胞之间的杂种细胞，并对其多代的特性和染色体组成进行了研究。通过酶学研究证实了它们的杂种性质。在1年的时间里，观察到染色体总数减少了10%至20%。SV40肿瘤抗原存在于杂种细胞中，并一直存在。对亲代细胞和杂种细胞进行了刀豆球蛋白A凝集、软琼脂中生长以及血清需求方面的研究。转化细胞的这些生长和表面特征出现在杂种细胞中。
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