9-β-D-阿拉伯呋喃糖基腺嘌呤对劳氏肉瘤病毒转化的大鼠细胞中核DNA合成的选择性抑制作用。
Selective inhibition of nuclear DNA synthesis by 9- -D-arabinofuranosyl adenine in rat cells transformed by Rous sarcoma virus.
作者信息
Shipman C, Smith S H, Drach J C
出版信息
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1753-7. doi: 10.1073/pnas.69.7.1753.
Abstract
The drug, 9-beta-D-arabinofuranosyl adenine, selectively inhibits the synthesis of nuclear DNA without affecting extrachromosomal DNA synthesis in rat cells transformed by Rous sarcoma virus (B-mix K-44/6). The inhibition was linear with respect to drug concentration over the range of 37-600 muM. Mitosis and total synthesis of DNA per cell were also depressed. DNA synthesis was determined by measurement of [(3)H]thymidine incorporation into DNA. Covalently-closed circular DNA was extracted by the Hirt procedure and separated from residual chromosomal DNA by buoyant density gradient ultracentrifugation in CsCl-propidium diiodide. On the basis of buoyant density and sedimentation velocity centrifugation, the covalently-closed circular DNA formed in the presence of the drug was indistinguishable from that formed in its absence.
摘要
药物9-β-D-阿拉伯呋喃糖基腺嘌呤可选择性抑制核DNA的合成，而不影响劳斯肉瘤病毒（B-mix K-44/6）转化的大鼠细胞中的染色体外DNA合成。在37 - 600μM范围内，抑制作用与药物浓度呈线性关系。每个细胞的有丝分裂和DNA总合成也受到抑制。通过测量[³H]胸苷掺入DNA来测定DNA合成。采用Hirt方法提取共价闭合环状DNA，并通过在CsCl-碘化丙啶中进行浮力密度梯度超速离心将其与残留的染色体DNA分离。基于浮力密度和沉降速度离心，在药物存在下形成的共价闭合环状DNA与不存在药物时形成的DNA无法区分。
相似文献
1
Selective inhibition of nuclear DNA synthesis by 9- -D-arabinofuranosyl adenine in rat cells transformed by Rous sarcoma virus.9-β-D-阿拉伯呋喃糖基腺嘌呤对劳氏肉瘤病毒转化的大鼠细胞中核DNA合成的选择性抑制作用。
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1753-7. doi: 10.1073/pnas.69.7.1753.
2
Temperature-dependent formation of dimers and oligomers of mitochondrial DNA in cells transformed by a thermosensitive mutant of Rous sarcoma virus.劳斯肉瘤病毒温度敏感突变体转化的细胞中线粒体DNA二聚体和寡聚体的温度依赖性形成。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3739-43. doi: 10.1073/pnas.70.12.3739.
3
Metabolic requirements for infection by Rous sarcoma virus. IV. Virus reproduction and cellular transformation without cellular division.
Virology. 1972 May;48(2):494-501. doi: 10.1016/0042-6822(72)90060-8.
4
Abnormal DNA patterns in animal mitochondria: ethidium bromide-induced breakdown of closed circular DNA and conditions leading to oligomer accumulation.动物线粒体中的异常DNA模式：溴化乙锭诱导的闭环DNA断裂及导致寡聚物积累的条件。
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1926-33. doi: 10.1073/pnas.67.4.1926.
5
Selective lysis of cells transformed by Rous Sarcoma virus.劳氏肉瘤病毒转化细胞的选择性裂解
Virology. 1971 Jul;45(1):172-81. doi: 10.1016/0042-6822(71)90124-3.
6
Differential effects of ethidium bromide on mitochondrial and nuclear DNA synthesis in vivo in cultured mammalian cells.溴化乙锭对培养的哺乳动物细胞体内线粒体和核DNA合成的不同影响。
Exp Cell Res. 1972 May;72(1):211-22. doi: 10.1016/0014-4827(72)90583-6.
7
Transformation by a temperature sensitive mutant of Rous sarcoma virus in the absence of serum.在无血清情况下由劳氏肉瘤病毒的温度敏感突变体介导的转化
J Gen Virol. 1975 May;27(2):127-34. doi: 10.1099/0022-1317-27-2-127.
8
Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts.劳斯肉瘤病毒特异性脱氧核糖核酸在允许性宿主和非允许性宿主感染后的整合。
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3067-71. doi: 10.1073/pnas.70.11.3067.
9
Cell transformation induced by Rous sarcoma virus: analysis of density dependence.劳氏肉瘤病毒诱导的细胞转化：密度依赖性分析
Virology. 1971 Nov;46(2):200-20.
10
Inhibition of development of transformation by Rous sarcoma virus in cultures of high cell density.在高细胞密度培养物中对劳氏肉瘤病毒转化发育的抑制作用。
J Cell Physiol. 1974 Apr;83(2):203-10. doi: 10.1002/jcp.1040830206.
引用本文的文献
1
Establishment of a murine cell line resistant to arabinosyladenine and devoid of adenosine deaminase activity.建立对阿糖腺苷耐药且缺乏腺苷脱氨酶活性的小鼠细胞系。
Antimicrob Agents Chemother. 1983 Dec;24(6):947-9. doi: 10.1128/AAC.24.6.947.
2
Complete moles have paternal chromosomes but maternal mitochondrial DNA.完全性葡萄胎具有父系染色体，但线粒体DNA来自母系。
Hum Genet. 1982;61(2):145-7. doi: 10.1007/BF00274205.
3
Temperature-dependent formation of dimers and oligomers of mitochondrial DNA in cells transformed by a thermosensitive mutant of Rous sarcoma virus.劳斯肉瘤病毒温度敏感突变体转化的细胞中线粒体DNA二聚体和寡聚体的温度依赖性形成。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3739-43. doi: 10.1073/pnas.70.12.3739.
4
Probable synchronous replication of mitochondrial DNA in cultures of chick embryo fibroblasts.
Mol Biol Rep. 1975 Dec;2(4):327-33. doi: 10.1007/BF00357020.
5
Antiviral activity of arabinosyladenine and arabinosylhypoxanthine in herpes simplex virus-infected KB cells: selective inhibition of viral deoxyribonucleic acid synthesis in synchronized suspension cultures.阿糖腺苷和阿糖次黄嘌呤在单纯疱疹病毒感染的KB细胞中的抗病毒活性：在同步悬浮培养物中对病毒脱氧核糖核酸合成的选择性抑制
Antimicrob Agents Chemother. 1976 Jan;9(1):120-7. doi: 10.1128/AAC.9.1.120.
本文引用的文献
1
The toxicity of 9-beta-D-arabinofuranosyladenine to purine-requiring Escherichia coli.9-β-D-阿拉伯呋喃糖基腺嘌呤对需要嘌呤的大肠杆菌的毒性。
Biochim Biophys Acta. 1962 May 21;59:468-71. doi: 10.1016/0006-3002(62)90198-1.
2
METABOLIC EFFECTS OF 9-D-ARABINOSYLPURINES IN ASCITES TUMOR CELLS.9-D-阿拉伯糖基嘌呤对腹水肿瘤细胞的代谢作用
Cancer Res. 1964 Feb;24:312-8.
3
Effects of arabinonucleotides on ribonucleotide reduction by an enzyme system from rat tumor.阿拉伯核苷酸对大鼠肿瘤酶系统还原核糖核苷酸的影响。
J Biol Chem. 1967 May 10;242(9):2116-8.
4
Some effects of D-arabinosyl nucleosides on polymer syntheses in mouse fibroblasts.D-阿拉伯糖基核苷对小鼠成纤维细胞中聚合物合成的某些影响。
Cancer Res. 1966 Dec;26(12):2444-50.
5
A proposed mechanism for the action of 9-beta-D-arabinofuranosyladenine as an inhibitor of the growth of some ascites cells.9-β-D-阿拉伯呋喃糖基腺嘌呤作为某些腹水细胞生长抑制剂的作用机制推测。
Can J Biochem. 1966 Jan;44(1):19-26. doi: 10.1139/o66-003.
6
Purified cytoplasmic DNA from HeLa cells: resistance to inhibition by hydroxyurea.
Biochem Biophys Res Commun. 1969 Apr 29;35(2):249-57. doi: 10.1016/0006-291x(69)90275-7.
7
Evaluation of 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES) as a tissue culture buffer.评估4-（2-羟乙基）-1-哌嗪乙磺酸（HEPES）作为组织培养缓冲液的性能。
Proc Soc Exp Biol Med. 1969 Jan;130(1):305-10. doi: 10.3181/00379727-130-33543.
8
9
Arabinosyladenine and arabinosylhypoxanthine metabolism in murine tumor cells.鼠肿瘤细胞中阿糖腺苷和阿糖次黄嘌呤的代谢
Can J Biochem. 1970 Jan;48(1):75-8. doi: 10.1139/o70-013.
10
Phosphorylation of D-Arabinosyl adenine by Plasmodium berghei and its partial protection of mice against malaria.伯氏疟原虫对D-阿拉伯糖基腺嘌呤的磷酸化作用及其对小鼠疟疾的部分保护作用。
Nature. 1970 Dec 26;228(5278):1300-1. doi: 10.1038/2281300a0.
Zotero 插件