体外胰腺淀粉酶释放的调控：离子、环磷酸腺苷和秋水仙碱的作用

The time course and concentration-response relationship of amylase release from pieces of guinea-pig pancreas in vitro in response to bethanechol and pancreozymin was determined.2. Removal of Ca(++) from the medium had no effect on basal amylase release but abolished the stimulating effect on release of bethanechol.3. Elevation of the concentration of Mg(++) in the medium increased basal amylase release and reduced the response to bethanechol.4. Elevation of the concentration of K(+) in the medium increased amylase release; this effect was blocked by a concentration of atropine which blocked also the response to bethanechol.5. Cyclic AMP, dibutyryl cyclic AMP and theophylline failed to stimulate amylase release. Pancreatic cyclic AMP concentrations were found not to be increased by bethanechol, pancreozymin or an elevated concentration of K(+) in the medium.6. Colchicine had no effect on basal amylase release or the response to bethanechol or pancreozymin.7. It is concluded that the coupling of stimulus to secretion involves ionic control but that neither cyclic AMP production nor microtubular mechanisms play a major role in controlling exocytosis in the pancreatic acinar cell. These findings are discussed in relation to the stimulus-secretion coupling processes in other cells.

测定了豚鼠胰腺组织块在体外对氨甲酰甲胆碱和促胰液素反应时淀粉酶释放的时间进程和浓度 - 反应关系。
从培养基中去除Ca(++)对基础淀粉酶释放无影响，但消除了氨甲酰甲胆碱对释放的刺激作用。
培养基中Mg(++)浓度升高增加了基础淀粉酶释放，并降低了对氨甲酰甲胆碱的反应。
培养基中K(+)浓度升高增加了淀粉酶释放；这种作用被一定浓度的阿托品阻断，该浓度的阿托品也阻断了对氨甲酰甲胆碱的反应。
环磷酸腺苷、二丁酰环磷酸腺苷和茶碱未能刺激淀粉酶释放。发现氨甲酰甲胆碱、促胰液素或培养基中升高的K(+)浓度均未增加胰腺环磷酸腺苷浓度。
秋水仙碱对基础淀粉酶释放或对氨甲酰甲胆碱或促胰液素的反应无影响。
得出结论，刺激与分泌的偶联涉及离子控制，但环磷酸腺苷生成和微管机制在胰腺腺泡细胞控制胞吐作用中均不发挥主要作用。结合其他细胞中的刺激 - 分泌偶联过程对这些发现进行了讨论。

Control of pancreatic amylase release in vitro: effects of ions, cyclic AMP, and colchicine.