Ushimi C, Henson J B, Gorham J R
Infect Immun. 1972 Jun;5(6):890-5. doi: 10.1128/iai.5.6.890-895.1972.
Primary horse leukocyte cultures were inoculated with 2 or 10 50% tissue culture infective doses (TCID(50)) of equine infectious anemia (EIA) virus per cell, and the titer of cell-associated and fluid-phase virus was determined from 1 to 72 hr postinoculation (PI). Cover slips were collected from 4 to 72 hr PI and stained for EIA viral antigen by the indirect immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 18 to 24 hr and reached peak titers of approximately 10(4.5) to 10(6) TCID(50)/0.5 ml from 48 to 72 hr PI. The fluid phase contained 10(1) to 10(2) TCID(50)/0.5 ml more virus than the cells. Viral antigen was first detected by FA from 18 to 24 hr PI. Approximately 75% of the cells contained antigen in their cytoplasm 72 hr PI. The FA technique is a sensitive method for detecting EIA virus in horse leukocyte cultures.
将原代马白细胞培养物以每细胞2或10个50%组织培养感染剂量（TCID₅₀）的马传染性贫血（EIA）病毒接种，并在接种后1至72小时（PI）测定细胞相关病毒和液相病毒的滴度。在接种后4至72小时收集盖玻片，并通过间接免疫荧光（FA）技术对EIA病毒抗原进行染色。在约18至24小时的潜伏期后检测到病毒复制，并且在接种后48至72小时达到约10⁴·⁵至10⁶ TCID₅₀/0.5 ml的峰值滴度。液相中的病毒比细胞中的病毒多10¹至10² TCID₅₀/0.5 ml。通过FA在接种后18至24小时首次检测到病毒抗原。在接种后72小时，约75%的细胞在其细胞质中含有抗原。FA技术是检测马白细胞培养物中EIA病毒的一种灵敏方法。
