Equine Infectious Anemia Research Division, National Institute of Animal Health, Kodaira, Tokyo, Japan.
Infect Immun. 1971 Mar;3(3):373-7. doi: 10.1128/iai.3.3.373-377.1971.
Antigenicity of purified equine infectious anemia (EIA) virus was examined by immunodiffusion against sera obtained from horses experimentally infected with EIA virus. The purified virus reacted with the infected horse serum, and virus-specific precipitating antibody was demonstrated. Furthermore, it was found that purified EIA virus reacted against the serum of horses infected with all strains of EIA virus which were antigenically different from one another. From the result, group-specific components of the virus rather than strain-specific ones were considered to be involved in the reaction. Serological reactivity was lost by adding antiserum from the infected horse to the antigen. The precipitating antibody usually appeared in the serum 1 to 2 weeks after the first febrile attack of EIA and remained for a longer period. Some characteristics of the purified antigen and specificity of the reaction for EIA are described.
用免疫扩散法对用马传染性贫血病毒（EIA 病毒）人工感染马所获得的血清进行了检验，以检查提纯的 EIA 病毒的抗原性。提纯的病毒与感染马的血清发生反应，并证明存在病毒特异性沉淀抗体。此外，还发现提纯的 EIA 病毒与在抗原性上彼此不同的所有 EIA 病毒株感染的马血清发生反应。由此认为，与反应有关的是病毒的属特异性成分，而不是株特异性成分。在抗原中加入感染马的抗血清，可使血清学反应性丧失。沉淀抗体通常在 EIA 的首次发热后 1 至 2 周出现在血清中，并持续较长时间。本文还描述了提纯抗原的某些特性及其对 EIA 的反应特异性。
