影响次黄嘌呤：鸟嘌呤磷酸核糖转移酶结构的突变在培养的中国仓鼠细胞中。
Mutations affecting the structure of hypoxanthine: guanine phosphoribosyltransferase in cultured Chinese hamster cells.
作者信息
Beaudet A L, Roufa D J, Caskey C T
出版信息
Proc Natl Acad Sci U S A. 1973 Feb;70(2):320-4. doi: 10.1073/pnas.70.2.320.
Abstract
14 Clones resistant to 8-azaguanine, isolated from mutagenically treated cultured cells from Chinese hamsters, were tested for loss of enzymic and immunological activities of hypoxanthine: guanine phosphoribosyltransferase. Three of the clones had no enzymic activity but reacted strongly with antiserum prepared against the enzyme (CRM(+)), indicating the presence of a defective enzyme protein, probably caused by a mutation in its structural gene. The other 11 mutants had little or no enzymic or immunologic activity. Revertant clones with positive enzymic and crossreacting immunologic activities were derived from both classes of mutants. The antiserum has been used for rapid purification of the phosphoribosyltransferase from Chinese hamster cells by immunoadsorbant affinity chromatography.
摘要
从经诱变处理的中国仓鼠培养细胞中分离出14个对8-氮杂鸟嘌呤有抗性的克隆，检测它们次黄嘌呤：鸟嘌呤磷酸核糖转移酶的酶活性和免疫活性丧失情况。其中3个克隆没有酶活性，但与针对该酶制备的抗血清发生强烈反应（CRM(+)），表明存在有缺陷的酶蛋白，可能是由其结构基因突变所致。另外11个突变体几乎没有或完全没有酶活性和免疫活性。具有阳性酶活性和交叉反应免疫活性的回复克隆源自这两类突变体。该抗血清已用于通过免疫吸附亲和层析从中国仓鼠细胞中快速纯化磷酸核糖转移酶。
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Mutations affecting the structure of hypoxanthine: guanine phosphoribosyltransferase in cultured Chinese hamster cells.影响次黄嘌呤：鸟嘌呤磷酸核糖转移酶结构的突变在培养的中国仓鼠细胞中。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):320-4. doi: 10.1073/pnas.70.2.320.
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