军团病杆菌的细胞脂质

Cellular lipids of the Legionnaires' disease bacterium.

作者信息

Finnerty W R, Makula R A, Feeley J C

出版信息

Ann Intern Med. 1979 Apr;90(4):631-4. doi: 10.7326/0003-4819-90-4-631.

Abstract

We qualitatively and quantitatively analyzed the simple and complex lipids of 10 Legionnaires' disease bacteria. The phospholipids in decreasing order of concentration were phosphatidylcholine, phosphatidylethanolamine, cardiolipin, phosphatidylmonomethylethanolamine, phosphatidylglycerol, and phosphatidyldimethylethanolamine. The total phospholipids averaged 96 micromoles per gram dry cell weight. Phospholipid fatty acids were solely branched-chain fatty acids and were, in decreasing order of concentration, iso-C16:0, anteiso-C15:0, anteiso-C17:0, iso-C14:0, iso-C16:1, and an unidentified fatty acid. Neutral lipids identified were free fatty acid, ubiquinone, triglyceride, diglyceride, monoglyceride, and wax ester. Neutral lipid fatty acids consisted predominately of branched-chain fatty acids, normal fatty acids, and a minor unidentified fatty acid. Analysis of the cellular lipids of 10 Legionnaires' disease bacteria revealed an unusual and novel microorganism.

摘要

我们对10株嗜肺军团菌的简单脂质和复合脂质进行了定性和定量分析。磷脂浓度由高到低依次为磷脂酰胆碱、磷脂酰乙醇胺、心磷脂、磷脂酰单甲基乙醇胺、磷脂酰甘油和磷脂酰二甲基乙醇胺。总磷脂平均为每克干细胞重96微摩尔。磷脂脂肪酸均为支链脂肪酸，浓度由高到低依次为异-C16:0、anteiso-C15:0、anteiso-C17:0、异-C14:0、异-C16:1和一种未鉴定的脂肪酸。鉴定出的中性脂质有游离脂肪酸、泛醌、甘油三酯、甘油二酯、甘油单酯和蜡酯。中性脂质脂肪酸主要由支链脂肪酸、正常脂肪酸和少量未鉴定的脂肪酸组成。对10株嗜肺军团菌的细胞脂质分析揭示了一种不同寻常的新型微生物。