在鸡胚卵黄囊中生长的普氏立克次体的磷脂组成。
Phospholipid composition of Rickettsia prowazeki grown in chicken embryo yolk sacs.
作者信息
Winkler H H, Miller E T
出版信息
J Bacteriol. 1978 Oct;136(1):175-8. doi: 10.1128/jb.136.1.175-178.1978.
Abstract
The phospholipid composition and phospholipid fatty acid composition of purified Rickettsia prowazeki were determined. The lipid phosphorous content was 6.8 +/- 1.3 microgram/mg of total rickettsial protein. The major phospholipid was phosphatidylethanolamine (60 to 70%); phosphatidylglycerol constituted 20%, and phosphatidylcholine constituted 15%. Small amounts of phosphatidylserine and cardiolipin were detected. The principal fatty acids were 18:1, 16:1, and 16:0. The fatty acid composition of the phosphatidylcholine in the rickettsial extracts was very different than that of the other rickettsial phosphatides and very similar to that of normal yolk sac phosphatidylcholine. The specific of the phosphatidylcholine of rickettsiae grown in the presence of 32P was markedly lower than that of phosphatidylethanolamine and phosphatidylglycerol. It is suggested that the phosphatidylcholine in the rickettsial extract is yolk sac derived and either tightly absorbed or exchanged into the rickettsial membrane.
摘要
测定了纯化的普氏立克次体的磷脂组成和磷脂脂肪酸组成。脂质磷含量为每毫克立克次体总蛋白6.8±1.3微克。主要磷脂为磷脂酰乙醇胺（60%至70%）；磷脂酰甘油占20%，磷脂酰胆碱占15%。检测到少量的磷脂酰丝氨酸和心磷脂。主要脂肪酸为18:1、16:1和16:0。立克次体提取物中磷脂酰胆碱的脂肪酸组成与其他立克次体磷脂的脂肪酸组成非常不同，且与正常卵黄囊磷脂酰胆碱的脂肪酸组成非常相似。在32P存在下生长的立克次体的磷脂酰胆碱的比活性明显低于磷脂酰乙醇胺和磷脂酰甘油。提示立克次体提取物中的磷脂酰胆碱来源于卵黄囊，要么被紧密吸附，要么交换到立克次体膜中。
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