细胞松弛素B。六、培养的诺维科夫大鼠肝癌细胞对核苷转运的竞争性抑制作用。
Cytochalasin B. VI. Competitive inhibition of nucleoside transport by cultured Novikoff rat hepatoma cells.
作者信息
Plagemann P G, Estensen R D
出版信息
J Cell Biol. 1972 Oct;55(1):179-85. doi: 10.1083/jcb.55.1.179.
Abstract
Cytochalasin B competitively inhibits the transport of uridine and thymidine by Novikoff rat hepatoma cells growing in suspension culture with apparent K(i)'s of 2 and 6 microM, respectively, but has no effect on the intracellular phosphorylation of the nucleosides. Choline transport is not affected by cytochalasin B. Results from pulse-chase experiments indicate that cytochalasin B has no direct effect on the synthesis of RNA, DNA, or uridine diphosphate-sugars. The inhibition of uridine and thymidine incorporation into nucleic acids by cytochalasin B is solely the consequence of the inhibition of nucleoside transport.
摘要
细胞松弛素B竞争性抑制悬浮培养的诺维科夫大鼠肝癌细胞对尿苷和胸苷的转运，其表观抑制常数（K(i)）分别为2和6微摩尔，但对核苷的细胞内磷酸化没有影响。细胞松弛素B不影响胆碱的转运。脉冲追踪实验结果表明，细胞松弛素B对RNA、DNA或尿苷二磷酸糖的合成没有直接影响。细胞松弛素B抑制尿苷和胸苷掺入核酸完全是由于其对核苷转运的抑制作用。
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J Cell Biol. 1972 Oct;55(1):161-78. doi: 10.1083/jcb.55.1.161.
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