Davis W L, Goodman D B, Schuster R J
J Cell Biol. 1974 Dec;63(3):986-97. doi: 10.1083/jcb.63.3.986.
A combined physiological and morphological study of the effects of cytochalasin B (CB) on the toad urinary bladder has been carried out. CB inhibits the hydro-osmotic response to vasopressin without altering basal water permeability or diffusion, or the increase in (3)H(2)O diffusion observed after hormone addition. Although CB increases [(22)Na]-, [(36)Cl]-, and [(14)C]urea fluxes, and decreases transepithelial potential, no alteration in basal short-circuit current, the vasopressin-induced increase in this parameter, or [(14)C]inulin permeability occurs. In the absence of hormone, CB does not markedly alter the structure of the toad bladder. However, in the presence of vasopressin, CB induces the formation of large intracellular vacuoles. These results suggest a possible coupling of solute and water movement across the tissue.
对细胞松弛素B（CB）作用于蟾蜍膀胱的影响进行了生理和形态学的联合研究。CB抑制了对血管加压素的水渗透反应，而不改变基础水渗透性或扩散，也不改变添加激素后观察到的（3）H（2）O扩散增加。虽然CB增加了[（22）Na] -、[（36）Cl] -和[（14）C]尿素通量，并降低了跨上皮电位，但基础短路电流、血管加压素诱导的该参数增加或[（14）C]菊粉渗透性均未改变。在没有激素的情况下，CB不会显著改变蟾蜍膀胱的结构。然而，在血管加压素存在的情况下，CB会诱导形成大的细胞内液泡。这些结果表明溶质和水跨组织移动可能存在耦合。
