粗糙脉孢菌呼吸突变体的筛选。
Selection of respiratory mutants of Neurospora crassa.
作者信息
Edwards D L, Kwiecinski F, Horstmann J
出版信息
J Bacteriol. 1973 Apr;114(1):164-8. doi: 10.1128/jb.114.1.164-168.1973.
Abstract
A method is described that permits the isolation of mutants that are defective in mitochondrial respiration. The techniques of inositol-less death and overlay with 2,3,5-triphenyl-2H-tetrazolium chloride are utilized to select for mutant colonies. Colonies that survive inositol-less death and fail to reduce the tetrazolium dye are then tested polarographically for cyanide-sensitive respiration. A preliminary characterization of three mutants obtained by this method is presented. The mutants have been characterized by their cyanide-sensitive respiration rate, growth rate, the state III respiration rate of isolated mitochondria, inhibition of the respiration of isolated mitochondria by cyanide and antimycin A, and cytochrome spectra. All of the mutants described differ from the parent strain in some of these aspects.
摘要
本文描述了一种可分离线粒体呼吸缺陷型突变体的方法。利用无肌醇死亡技术和用2,3,5-三苯基-2H-四氮唑氯化物覆盖的方法来筛选突变菌落。然后对在无肌醇死亡中存活且不能还原四氮唑染料的菌落进行极谱法检测其对氰化物敏感的呼吸作用。本文给出了通过该方法获得的三个突变体的初步特征。这些突变体通过其对氰化物敏感的呼吸速率、生长速率、分离线粒体的状态III呼吸速率、氰化物和抗霉素A对分离线粒体呼吸的抑制作用以及细胞色素光谱进行了表征。所描述的所有突变体在这些方面中的某些方面与亲本菌株不同。
相似文献
1
Selection of respiratory mutants of Neurospora crassa.粗糙脉孢菌呼吸突变体的筛选。
J Bacteriol. 1973 Apr;114(1):164-8. doi: 10.1128/jb.114.1.164-168.1973.
2
Cyanide- and hydroxamate-resistant respiration in Neurospora crassa.粗糙脉孢菌中对氰化物和异羟肟酸耐药的呼吸作用
J Bacteriol. 1978 Mar;133(3):1130-4. doi: 10.1128/jb.133.3.1130-1134.1978.
3
Altered mitochondrial respiration in a chromosomal mutant of Neurospora crassa.粗糙脉孢菌染色体突变体中线粒体呼吸的改变。
J Bacteriol. 1973 Nov;116(2):610-8. doi: 10.1128/jb.116.2.610-618.1973.
4
GMP-stimulation of the cyanide-insensitive mitochondrial respiration in heat-shocked conidia of Neurospora crassa.在热激的粗糙脉孢菌分生孢子中，GMP对氰化物不敏感的线粒体呼吸的刺激作用。
Experientia. 1987 Apr 15;43(4):439-40. doi: 10.1007/BF01940445.
5
Studies of respiratory components and oxidative phosphorylation in mitochondria of mi-1 Neurospora crassa.粗糙脉孢菌mi-1线粒体中呼吸成分与氧化磷酸化的研究。
J Bacteriol. 1974 Feb;117(2):733-40. doi: 10.1128/jb.117.2.733-740.1974.
6
Effect of pyrrolnitrin on electron transport and oxidative phosphorylation in mitochondria isolated from Neurospora crassa.吡咯尼群对粗糙脉孢菌线粒体中电子传递和氧化磷酸化的影响。
J Bacteriol. 1972 Nov;112(2):1020-2. doi: 10.1128/jb.112.2.1020-1022.1972.
7
Cyanide-resistant respiration in Neurospora crassa.粗糙脉孢菌中的抗氰呼吸。
J Bacteriol. 1971 Dec;108(3):1087-96. doi: 10.1128/jb.108.3.1087-1096.1971.
8
Induction of cyanide-insensitive respiration in Neurospora crassa.粗糙脉孢菌中氰化物不敏感呼吸的诱导。
J Biol Chem. 1974 Jun 10;249(11):3551-6.
9
Cyanide-resistant respiration of a Neurospora crassa membrane mutant.粗糙脉孢菌膜突变体的抗氰呼吸
J Bacteriol. 1976 Apr;126(1):542-3. doi: 10.1128/jb.126.1.542-543.1976.
10
Growth, respiratory, and cytochrome characteristics of certain of the isoleucine-valine mutants of Neurospora crassa.粗糙脉孢菌某些异亮氨酸 - 缬氨酸突变体的生长、呼吸及细胞色素特性
Biochem Genet. 1974 Jul;12(1):39-49. doi: 10.1007/BF00487526.
引用本文的文献
1
Genetic interactions in the control of mitochondrial functions in Paramecium. I. Interactions between nuclear genes.草履虫线粒体功能控制中的基因相互作用。I. 核基因之间的相互作用
Mol Gen Genet. 1980;180(3):553-61. doi: 10.1007/BF00268060.
2
Isolation and characterization of light-insensitive mutants of Neurospora crassa.粗糙脉孢菌光不敏感突变体的分离与鉴定。
Genetics. 1983 May;104(1):11-21. doi: 10.1093/genetics/104.1.11.
3
Chromosomal loci of Neurospora crassa.粗糙脉孢菌的染色体位点。
Microbiol Rev. 1982 Dec;46(4):426-570. doi: 10.1128/mr.46.4.426-570.1982.
4
Isolation and biochemical analysis of Mucor bacilliformis monomorphic mutants.毛霉杆状单形突变体的分离与生化分析
J Bacteriol. 1983 Oct;156(1):264-72. doi: 10.1128/jb.156.1.264-272.1983.
5
Altered mitochondrial respiration in a chromosomal mutant of Neurospora crassa.粗糙脉孢菌染色体突变体中线粒体呼吸的改变。
J Bacteriol. 1973 Nov;116(2):610-8. doi: 10.1128/jb.116.2.610-618.1973.
6
Nuclear mutants of Neurospora crassa temperature-sensitive for the synthesis of cytochrome aa3. I. Isolation and preliminary characterization.粗糙脉孢菌中对细胞色素aa3合成温度敏感的核突变体。I. 分离与初步表征。
Mol Gen Genet. 1978 Oct 25;166(1):15-23. doi: 10.1007/BF00379724.
7
Selection and characterization of nuclear mutations affecting mitochondria in Paramecium.草履虫中影响线粒体的核突变的筛选与鉴定
Mol Gen Genet. 1978 Jun 1;162(1):1-8. doi: 10.1007/BF00333844.
8
Selection of succinic dehydrogenase mutants of Neurospora crassa.粗糙脉孢菌琥珀酸脱氢酶突变体的筛选。
J Bacteriol. 1979 Feb;137(2):900-4. doi: 10.1128/jb.137.2.900-904.1979.
9
Cyanide- and hydroxamate-resistant respiration in Neurospora crassa.粗糙脉孢菌中对氰化物和异羟肟酸耐药的呼吸作用
J Bacteriol. 1978 Mar;133(3):1130-4. doi: 10.1128/jb.133.3.1130-1134.1978.
10
Nuclear cytochrome-deficient mutants of Neurospora crassa: isolation, characterization, and genetic mapping.粗糙脉孢菌的细胞核细胞色素缺陷型突变体：分离、表征及遗传图谱构建。
Mol Gen Genet. 1977 Jun 24;153(3):247-57. doi: 10.1007/BF00431590.
本文引用的文献
1
Cytoplasmic Mutants Selected from Continuously Growing Cultures of NEUROSPORA CRASSA.从粗糙脉孢菌连续生长培养物中筛选出的细胞质突变体。
Genetics. 1969 Mar;61(3):643-59. doi: 10.1093/genetics/61.3.643.
2
A Case of "Maternal" Inheritance in Neurospora Crassa.粗糙脉孢菌中“母体”遗传的一个案例。
Proc Natl Acad Sci U S A. 1952 May;38(5):442-9. doi: 10.1073/pnas.38.5.442.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
Cytochromes and respiratory activities in some slow growing strains of Neurospora.
J Biol Chem. 1954 May;208(1):241-9.
5
Studies on the respiratory system of the poky strain of Neurospora.关于粗糙脉孢菌迟缓菌株呼吸系统的研究。
J Biol Chem. 1953 Nov;205(1):423-33.
6
Cytochromes and the succinic acid oxidase system of poky strains of Neurospora.粗糙脉孢菌迟缓菌株的细胞色素与琥珀酸氧化酶系统
J Biol Chem. 1953 Feb;200(2):819-26.
7
An altered cytochrome oxidase in a cytoplasmic mutant of Neurospora.粗糙脉孢菌细胞质突变体中一种改变的细胞色素氧化酶。
FEBS Lett. 1969 Aug;4(3):193-196. doi: 10.1016/0014-5793(69)80232-2.
8
A cytoplasmic variant of Neurospora crassa.粗糙脉孢菌的一种细胞质变体。
Genetics. 1966 Aug;54(2):551-65. doi: 10.1093/genetics/54.2.551.
9
Studies on a cytoplasmic character in Neurospora crassa.粗糙脉孢菌细胞质性状的研究。
J Cell Biol. 1965 Aug;26(2):413-25. doi: 10.1083/jcb.26.2.413.
10
Acetate-nonutilizing mutants of Neurospora rassa. II. Biochemical deficiencies and the roles of certain enzymes.粗糙脉孢菌的乙酸盐非利用突变体。II. 生化缺陷及某些酶的作用
J Bacteriol. 1968 Mar;95(3):1063-8. doi: 10.1128/jb.95.3.1063-1068.1968.
Zotero 插件