Ruiz-Herrera J, Ruiz A, Lopez-Romero E
J Bacteriol. 1983 Oct;156(1):264-72. doi: 10.1128/jb.156.1.264-272.1983.
Fourteen stable mutants of Mucor bacilliformis which grew yeastlike under both aerobic and anaerobic conditions were isolated after treatment of growing mycelium with N-methyl-N'-nitro-N-nitrosoguanidine. Biochemical characterization of the mutants included determination of growth in different carbon and nitrogen sources, determination of sensitivity of respiration to cyanide and salicylhydroxamate, analysis of cytochrome spectra, determination of glutamate dehydrogenases, glutamine synthase, and ornithine decarboxylase activities, and measurement of cyclic AMP levels. Data showed that all mutants were defective in some aspect of oxidative metabolism and had low levels of ornithine decarboxylase, whereas other characters were variable. It was concluded that morphological transition in M. bacilliformis is probably associated with mitochondrial functions and expression of ornithine decarboxylase, but may be independent of cyclic AMP and glutamate dehydrogenase levels. The importance of genetic studies in the analysis of dimorphism is stressed.
在用N-甲基-N'-硝基-N-亚硝基胍处理生长中的菌丝体后，分离出了14株在需氧和厌氧条件下均呈酵母样生长的稳定的杆状毛霉突变体。对这些突变体的生化特性进行了表征，包括测定在不同碳源和氮源中的生长情况、测定呼吸对氰化物和水杨羟肟酸的敏感性、分析细胞色素光谱、测定谷氨酸脱氢酶、谷氨酰胺合成酶和鸟氨酸脱羧酶的活性，以及测量环磷酸腺苷水平。数据表明，所有突变体在氧化代谢的某些方面存在缺陷，鸟氨酸脱羧酶水平较低，而其他特征则各不相同。得出的结论是，杆状毛霉的形态转变可能与线粒体功能和鸟氨酸脱羧酶的表达有关，但可能与环磷酸腺苷和谷氨酸脱氢酶水平无关。强调了遗传研究在双态性分析中的重要性。
