黏液病毒对血细胞吸附作用的定量评估：病毒血细胞吸附试验。
Quantitative assessment of hemadsorption by myxoviruses: virus hemadsorption assay.
作者信息
Hahon N, Booth J A, Eckert H L
出版信息
Appl Microbiol. 1973 Apr;25(4):595-600. doi: 10.1128/am.25.4.595-600.1973.
Abstract
The standardization and quantitative evaluation of an assay for myxoviruses, based on the enumeration of individual infected clone 1-5C-4 cells manifesting hemadsorption within 24 h of infection, are described. Hemadsorption was detectable earlier than immunofluorescence in infected cells or hemagglutinins in culture medium. The relationship between virus concentration and cells exhibiting hemadsorption was linear. The assay was highly precise, sensitive, and reproducible.
摘要
本文描述了一种基于对感染后24小时内表现出血细胞吸附的单个感染克隆1-5C-4细胞进行计数的粘病毒检测方法的标准化和定量评估。在感染细胞中，血细胞吸附比免疫荧光更早可检测到，在培养基中比血凝素更早可检测到。病毒浓度与表现出血细胞吸附的细胞之间的关系是线性的。该检测方法具有高度的精确性、敏感性和可重复性。
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