Hahon N, Stewart J D, Eckert H L
Appl Microbiol. 1973 Apr;25(4):601-5. doi: 10.1128/am.25.4.601-605.1973.
A quantitative hemadsorption-inhibition test was developed to estimate myxovirus serum antibodies within 24 h by determining the serum dilution inhibiting hemadsorption in 50% of the infected cells. The test depends on the interactions of virus-infected cell monolayers with antiviral serum and of the resultant complexes with antiimmunoglobulin G serum. The incorporation of species-specific anti-immunoglobulin G serum into the test significantly increased sensitivity.
已开发出一种定量血细胞吸附抑制试验,通过确定抑制50%受感染细胞血细胞吸附的血清稀释度,在24小时内估算黏液病毒血清抗体。该试验依赖于病毒感染的细胞单层与抗病毒血清的相互作用,以及所得复合物与抗免疫球蛋白G血清的相互作用。在试验中加入种属特异性抗免疫球蛋白G血清可显著提高灵敏度。