流感抗免疫球蛋白G血凝抑制试验的进一步研究
Further studies on the anti-immunoglobulin G hemagglutination-inhibition test for influenza.
作者信息
Hahon N, Booth J A, Eckert H L
出版信息
Appl Microbiol. 1972 Mar;23(3):485-9. doi: 10.1128/am.23.3.485-489.1972.
Abstract
Incorporating species-specific anti-immunoglobulin G (IgG) serum into the hemagglutination-inhibition (HI) test for influenza markedly increased sensitivity without loss of specificity. The effectiveness of anti-IgG serum for augmenting antibody titers may be influenced by the variable potency of commercial anti-IgG preparations. Maximal enhancement of HI titers was achieved when anti-IgG serum and virus-antiserum mixtures were incubated at 23 C for 10 min. Precision and reproducibility of the test were within acceptable limits. Other conditions likely to affect the test were investigated.
摘要
将种属特异性抗免疫球蛋白G（IgG）血清加入流感血凝抑制（HI）试验中，可显著提高敏感性且不失特异性。商业抗IgG制剂效力的差异可能会影响抗IgG血清提高抗体滴度的效果。当抗IgG血清与病毒抗血清混合物在23℃孵育10分钟时，HI滴度可实现最大增强。该试验的精密度和可重复性在可接受范围内。还对其他可能影响该试验的条件进行了研究。
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本文引用的文献
1
Serum Neutralization Anti-IgG Test for Psittacosis.鹦鹉热血清中和抗 IgG 试验。
Infect Immun. 1970 Jul;2(1):7-10. doi: 10.1128/iai.2.1.7-10.1970.
2
Detection of Q fever antibodies by the anti-globulin sensitization test.通过抗球蛋白致敏试验检测Q热抗体。
Lancet. 1951 Jul 7;2(6671):15-7. doi: 10.1016/s0140-6736(51)93452-6.
3
Serum agglutinins in brucellosis.
Nature. 1951 Apr 7;167(4249):558-9. doi: 10.1038/167558b0.
4
ENHANCEMENT OF MYCOPLASMA AGGLUTINATION TITERS BY USE OF ANTI-GLOBULIN.使用抗球蛋白提高支原体凝集效价。
Proc Soc Exp Biol Med. 1964 Jul;116:608-10. doi: 10.3181/00379727-116-29318.
5
Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。
J Immunol. 1962 Mar;88:320-9.
6
Kaolin treatment of sera for removal of nonspecific inhibitors to Asian strains of influenza virus.用高岭土处理血清以去除对亚洲流感病毒株的非特异性抑制剂。
Proc Soc Exp Biol Med. 1960 Feb;103:425-7. doi: 10.3181/00379727-103-25545.
7
The action of trypsin on normal serum inhibitors of influenza virus agglutination.胰蛋白酶对流感病毒凝集正常血清抑制剂的作用。
Br J Exp Pathol. 1953 Apr;34(2):152-8.
8
Infectious virus-antibody complex in the blood of chronically infected mice.慢性感染小鼠血液中的传染性病毒-抗体复合物。
J Exp Med. 1966 Jul 1;124(1):81-97. doi: 10.1084/jem.124.1.81.
9
Hydrogen ion buffers for biological research.用于生物学研究的氢离子缓冲剂。
Biochemistry. 1966 Feb;5(2):467-77. doi: 10.1021/bi00866a011.
10
[Enhancement of neutralization of vaccinia virus by antiglobulin].[抗球蛋白增强牛痘病毒中和作用]
Arch Gesamte Virusforsch. 1970;31(1):28-32.
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