同位素标记对制备型超速离心机中病毒DNA浮力密度测定的影响。
Effect of isotopic label on buoyant density determinations of viral DNA in the preparative ultracentrifuge.
作者信息
Cassai E, Bachenheimer S
出版信息
J Virol. 1973 Apr;11(4):610-3. doi: 10.1128/JVI.11.4.610-613.1973.
Abstract
The buoyant density of herpes simplex virus DNA may be significantly increased by isotopic labeling, particularly with (14)C. The apparent increase in buoyant density may be readily calculated from the specific activity of the DNA and is easily demonstrated by isopycnic centrifugation in both the preparative and analytical centrifuge.
摘要
单纯疱疹病毒DNA的浮力密度可通过同位素标记，特别是用（14）C进行标记而显著增加。浮力密度的明显增加可根据DNA的比活性轻易计算出来，并且在制备型离心机和分析型离心机中通过等密度离心很容易得到证实。
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本文引用的文献
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