环腺苷酸3',5'-单磷酸在大肠杆菌半乳糖操纵子体内表达中的作用。
Role of cyclic adenosine 3',5'-monophosphate in the in vivo expression of the galactose operon of Escherichia coli.
作者信息
Rothman-Denes L B, Hesse J E, Epstein W
出版信息
J Bacteriol. 1973 Jun;114(3):1040-4. doi: 10.1128/jb.114.3.1040-1044.1973.
Abstract
Studies of levels of galactokinase in Escherichia coli with mutations affecting synthesis of, or response to, cyclic adenosine 3',5'-monophosphate show that this nucleotide does not play a major role in expression of the galactose operon, causing at most a twofold stimulation. The discrepancy between our in vivo results and the marked stimulation by cyclic adenosine 3',5'-monophosphate in in vitro systems indicates that current cell-free systems lack a factor which allows efficient expression of the galactose operon even in the absence of cyclic adenosine 3',5'-monophosphate or of the binding protein for this nucleotide.
摘要
对大肠杆菌中半乳糖激酶水平的研究表明，那些影响环腺苷酸（3',5'-单磷酸）合成或对其反应的突变，这种核苷酸在半乳糖操纵子的表达中并不起主要作用，最多引起两倍的刺激。我们的体内实验结果与环腺苷酸（3',5'-单磷酸）在体外系统中的显著刺激之间的差异表明，目前的无细胞系统缺乏一种即使在没有环腺苷酸（3',5'-单磷酸）或其结合蛋白的情况下也能有效表达半乳糖操纵子的因子。
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