半乳糖操纵子表达的调控：葡萄糖效应及环腺苷酸 3',5'-单磷酸的作用
Regulation of galactose operon expression: glucose effects and role of cyclic adenosine 3',5'-monophosphate.
作者信息
Joseph E, Danchin A, Ullmann A
出版信息
J Bacteriol. 1981 Apr;146(1):149-54. doi: 10.1128/jb.146.1.149-154.1981.
Abstract
We studied the following two aspects of the glucose effect on galactose operon expression in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, we found that the galactose operon did not seem to exhibit catabolite repression. The only glucose effect on galactose operon expression which we observed was inducer exclusion, as shown by the existence of diauxic growth in the presence of glucose and galactose. This diauxie was not relieved by cyclic adenosine 3',5'-monophosphate. Cyclic adenosine 3',5'-monophosphate did not seem to be an antagonist of any glucose effect on galactose operon expression; its only effect was to stimulate promoter-distal gene expression.
摘要
我们研究了葡萄糖对大肠杆菌K-12中半乳糖操纵子表达影响的以下两个方面：分解代谢物阻遏和诱导物排除。通过使用诱导型和组成型菌株并测量启动子近端酶的合成速率，我们发现半乳糖操纵子似乎并未表现出分解代谢物阻遏。我们观察到的葡萄糖对半乳糖操纵子表达的唯一影响是诱导物排除，这在葡萄糖和半乳糖存在下出现的双相生长中得以体现。这种双相生长不会被3',5'-环腺苷酸缓解。3',5'-环腺苷酸似乎并不是葡萄糖对半乳糖操纵子表达产生的任何影响的拮抗剂；它唯一的作用是刺激启动子远端基因的表达。
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