Stephenson J R, Wilsnack R E, Aaronson S A
J Virol. 1973 Jun;11(6):893-9. doi: 10.1128/JVI.11.6.893-899.1973.
A radioimmunoassay has been developed for detection of avian C-type virus (30,000 mol wt) group-specific (gs) antigen. The method is 10- to 1,000-fold more sensitive than immunological methods previously available. By the radioimmunoassay technique, normal chicken embryo cells, which have previously been classified as gs negative or weakly gs positive, contain clearly detectable amounts of gs antigen. The assay has been used to study the effect of chemical induction and superinfection by mammalian C-type viruses on the expression of avian gs antigen in mammalian cells nonproductively transformed by avian sarcoma viruses.
已开发出一种放射免疫测定法,用于检测禽C型病毒(分子量30,000)的群特异性(gs)抗原。该方法的灵敏度比以前可用的免疫方法高10至1000倍。通过放射免疫测定技术,以前被归类为gs阴性或弱gs阳性的正常鸡胚细胞含有明显可检测量的gs抗原。该测定法已用于研究化学诱导和哺乳动物C型病毒的超感染对禽肉瘤病毒非生产性转化的哺乳动物细胞中禽gs抗原表达的影响。