Reynolds F H, Hanson C A, Aaronson S A, Stephenson J R
J Virol. 1977 Jul;23(1):74-9. doi: 10.1128/JVI.23.1.74-79.1977.
Sensitive radioimmunoassays were developed for avian type C viral gag gene-coded proteins. These assays were used to examine the restriction to virus production by avian embryo cells and mammalian cells transformed by avian sarcoma viruses. The results indicate that although a high-molecular-weight primary translational product of the gag gene is expressed, its cleavage and processing are incomplete. Furthermore, analysis of intermediate cleavage products provided information regarding the order of sequences coding for the individual viral proteins within the avian type C viral gag gene.
已开发出用于检测禽C型病毒gag基因编码蛋白的灵敏放射免疫测定法。这些测定法用于检测禽胚胎细胞和被禽肉瘤病毒转化的哺乳动物细胞对病毒产生的限制作用。结果表明，尽管gag基因的高分子量初级翻译产物能够表达，但其切割和加工并不完整。此外，对中间切割产物的分析提供了有关禽C型病毒gag基因内各个病毒蛋白编码序列顺序的信息。
