Nakayama I, Nickerson P A
Am J Pathol. 1973 May;71(2):279-94.
Two morphologically distinct gonadotrophs have been identified in the anterior pituitary of rats bearing the Snell 494 tumor. Type 1 gonadotrophs first showed ultrastructural alterations at 4 weeks after implantation of the tumor. Numerous large 300 to 600 mmu granules appeared in type 1 cells. The opacity of the granules was variable, and they sometimes contained non-membrane-bounded spherical inclusions. These granules were dispersed among more electron-opaque, 200-mmu granules usually seen in gonadotrophs. A third granule approximately 500 to 600 mmu in diameter was apparently a lysosome-derived structure. Type 2 gonadotrophs first showed changes after 4 weeks, but the alterations were especially prominent by 8 to 10 weeks. No large granules characteristic of type 1 cells were seen in type 2 cells. By 8 to 10 weeks, very few 200-mmu granules were observed in the cytoplasm of type 2 cells. The endoplasmic reticulum was dilated in most type 2 cells; frequently, the cisternae were dilated considerably, forming large lake-like areas. Hypertrophic rough endoplasmic reticulum and the presence of intracisternal granules within cisternae of the rough reticulum suggest that type 2 cells were stimulated by the tumor. Changes in gonadotrophs were undoubtedly produced by the large amount of corticosterone secreted by the tumor. The corticosterone secreted by the tumor. The corticosterone may well act directly on the hypothalamo-hypophyseal axis or it could act indirectly on the ovary; ovarian steroids would then exert a feedback effect. The ovary was atrophic and did not contain maturing Graafian follicles or corpora lutea. In somatotrophs, the number of secretory granules increased, probably reflecting suppression of hormonal release by corticosterone.
在患有斯奈尔494肿瘤的大鼠垂体前叶中，已鉴定出两种形态不同的促性腺激素细胞。1型促性腺激素细胞在肿瘤植入后4周首次出现超微结构改变。1型细胞中出现了许多直径为300至600毫微米的大颗粒。这些颗粒的不透明度各不相同，有时还含有无膜包被的球形内含物。这些颗粒散布在促性腺激素细胞中通常可见的电子密度更高的200毫微米颗粒之间。第三种颗粒直径约为500至600毫微米，显然是一种溶酶体衍生结构。2型促性腺激素细胞在4周后首次出现变化，但在8至10周时变化尤为明显。在2型细胞中未见到1型细胞特有的大颗粒。到8至10周时，在2型细胞的细胞质中观察到的200毫微米颗粒非常少。大多数2型细胞的内质网扩张；池常常显著扩张，形成大的湖状区域。粗糙内质网肥大以及粗糙内质网池内存在池内颗粒表明2型细胞受到肿瘤刺激。促性腺激素细胞的变化无疑是由肿瘤分泌的大量皮质酮引起的。肿瘤分泌的皮质酮。皮质酮很可能直接作用于下丘脑 - 垂体轴，或者它可能间接作用于卵巢；然后卵巢类固醇会发挥反馈作用。卵巢萎缩，不含成熟的格拉夫卵泡或黄体。在生长激素细胞中，分泌颗粒的数量增加，这可能反映了皮质酮对激素释放的抑制作用。
