Depiazzi L J, Richards R B
Aust Vet J. 1979 Jan;55(1):25-8. doi: 10.1111/j.1751-0813.1979.tb09541.x.
Bacteroides nodosus was recovered from naturally occurring cases of virulent ovine footrot (VFR) and benign footrot (BFR) using an artificial culture medium which incorporated trypticase, arginine, serine and 5% agar. A degrading proteinase (DP) test was developed which measured the proteinase activity of broth cultures of B. nodosus for a period of time after organism death to assess the stability of the enzyme. The spectrophotometric measurement of the release of dye from a hide powder--azure conjugate by the action of proteinase provided an objective analysis of enzyme activity. The DP test differentiated VFR and BFR isolates and promises to be a useful laboratory method for the diagnosis of benign and virulent footrot in sheep.
使用一种含有胰蛋白酶、精氨酸、丝氨酸和5%琼脂的人工培养基，从自然发生的恶性绵羊蹄腐病（VFR）和良性蹄腐病（BFR）病例中分离出结节拟杆菌。开发了一种降解蛋白酶（DP）试验，该试验在细菌死亡后的一段时间内测量结节拟杆菌肉汤培养物的蛋白酶活性，以评估酶的稳定性。通过蛋白酶作用从皮粉-天青偶联物中释放染料的分光光度法测量提供了对酶活性的客观分析。DP试验能够区分VFR和BFR分离株，有望成为诊断绵羊良性和恶性蹄腐病的一种有用的实验室方法。
