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1
Identification of inorganic pyrophosphate in human platelets and its release on stimulation with thrombin.人血小板中无机焦磷酸的鉴定及其在凝血酶刺激下的释放。
J Clin Invest. 1973 Jul;52(7):1595-600. doi: 10.1172/JCI107336.
2
Secretion, subcellular localization and metabolic status of inorganic pyrophosphate in human platelets. A major constituent of the amine-storing granules.人血小板中无机焦磷酸的分泌、亚细胞定位及代谢状态。胺储存颗粒的主要成分。
Biochem J. 1980 Oct 15;192(1):99-105. doi: 10.1042/bj1920099.
3
Enhanced increases in cytosolic Ca2+ in ADP-stimulated platelets from patients with delta-storage pool deficiency--a possible indicator of interactions between granule-bound ADP and the membrane ADP receptor.δ-储存池缺乏症患者的ADP刺激血小板中胞质Ca2+增强增加——颗粒结合ADP与膜ADP受体之间相互作用的一个可能指标。
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4
Alterations in individual molecular species of human platelet phospholipids during thrombin stimulation: electrospray ionization mass spectrometry-facilitated identification of the boundary conditions for the magnitude and selectivity of thrombin-induced platelet phospholipid hydrolysis.凝血酶刺激过程中人类血小板磷脂单个分子种类的变化：电喷雾电离质谱法助力确定凝血酶诱导血小板磷脂水解的幅度和选择性的边界条件。
Biochemistry. 1996 May 7;35(18):5822-32. doi: 10.1021/bi952927v.
5
Measurement of inorganic pyrophosphate in biological fluids. Elevated levels in some patients with osteoarthritis, pseudogout, acromegaly, and uremia.生物体液中无机焦磷酸的测量。骨关节炎、假性痛风、肢端肥大症和尿毒症患者中部分患者的水平升高。
J Clin Invest. 1973 Aug;52(8):1863-70. doi: 10.1172/JCI107369.
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Thrombin-activated human platelets release two NAP-2 variants that stimulate polymorphonuclear leukocytes.凝血酶激活的人血小板释放出两种刺激多形核白细胞的NAP-2变体。
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Brain-derived neurotrophic factor is stored in human platelets and released by agonist stimulation.脑源性神经营养因子储存在人类血小板中，并通过激动剂刺激释放。
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Conditions influencing release of granule contents from human platelets in citrated plasma induced by ADP or the thrombin receptor activating peptide SFLLRN: direct measurement of percent release of beta-thromboglobulin and assessment by flow cytometry of P-selectin expression.影响由ADP或凝血酶受体激活肽SFLLRN诱导的枸橼酸盐血浆中人类血小板颗粒内容物释放的条件：β-血小板球蛋白释放百分比的直接测量及通过流式细胞术评估P-选择素表达
Am J Hematol. 1996 Aug;52(4):288-94. doi: 10.1002/(SICI)1096-8652(199608)52:4<288::AID-AJH8>3.0.CO;2-O.
9
Alterations of adenine nucleotide metabolism and function of blood platelets in patients with diabetes.糖尿病患者血小板中腺嘌呤核苷酸代谢及功能的改变
Diabetes. 2007 Feb;56(2):462-7. doi: 10.2337/db06-0390.
10
Interaction of Ca2+ and protein phosphorylation in the rabbit platelet release reaction.兔血小板释放反应中钙离子与蛋白质磷酸化的相互作用。
J Clin Invest. 1980 Feb;65(2):242-55. doi: 10.1172/JCI109666.

引用本文的文献

1
A Reference Range for Plasma Levels of Inorganic Pyrophosphate in Children Using the ATP Sulfurylase Method.采用三磷酸腺苷硫酸化酶法测定儿童血浆中无机焦磷酸的参考范围。
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ABCC6 prevents ectopic mineralization seen in pseudoxanthoma elasticum by inducing cellular nucleotide release.ABCC6 通过诱导细胞核苷酸释放来防止弹性假黄瘤中所见的异位矿化。
Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20206-11. doi: 10.1073/pnas.1319582110. Epub 2013 Nov 25.
3
Using a filtration technique to isolate platelet free plasma for assaying pyrophosphate.采用过滤技术分离无血小板血浆以检测焦磷酸盐。
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4
Intracellular substrates for the primer-unblocking reaction by human immunodeficiency virus type 1 reverse transcriptase: detection and quantitation in extracts from quiescent- and activated-lymphocyte subpopulations.人免疫缺陷病毒1型逆转录酶用于引物解封反应的细胞内底物：静息和活化淋巴细胞亚群提取物中的检测与定量
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5
Precursors of monoamine-storage organelles in developing megakaryocytes of the rat.大鼠发育中巨核细胞内单胺储存细胞器的前体
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6
Secretion, subcellular localization and metabolic status of inorganic pyrophosphate in human platelets. A major constituent of the amine-storing granules.人血小板中无机焦磷酸的分泌、亚细胞定位及代谢状态。胺储存颗粒的主要成分。
Biochem J. 1980 Oct 15;192(1):99-105. doi: 10.1042/bj1920099.
7
An improved method for the isolation of dense storage granules from human platelets.一种从人血小板中分离致密储存颗粒的改进方法。
J Cell Biol. 1978 May;77(2):389-99. doi: 10.1083/jcb.77.2.389.

本文引用的文献

1
THE ESTIMATION OF 5-HYDROXYTRYPTAMINE IN HUMAN BLOOD.人体血液中5-羟色胺的测定
Biochem Pharmacol. 1964 Sep;13:1351-7. doi: 10.1016/0006-2952(64)90235-7.
2
THE SPECIFIC PRECIPITATION OF ORTHOPHOSPHATE AND SOME BIOCHEMICAL APPLICATIONS.正磷酸盐的特异性沉淀及一些生化应用
J Biol Chem. 1964 Jul;239:2360-4.
3
DIRECT COUNTING OF PLATELETS.血小板直接计数
Am J Med Technol. 1964 May-Jun;30:205-7.
4
An improved method for the micro-determination of inorganic phosphate in small volumes of biological fluids.一种用于微量测定小体积生物流体中无机磷酸盐的改进方法。
J Med Lab Technol. 1963 Apr;20:97-103.
5
An easy method to determine the serotonin content of human platelets.一种测定人体血小板中血清素含量的简便方法。
J Clin Pathol. 1962 Mar;15(2):191-3. doi: 10.1136/jcp.15.2.191.
6
Presence of pyrophosphatase in platelets.血小板中焦磷酸酶的存在。
Acta Haematol. 1954 Aug;12(2):81-6. doi: 10.1159/000204604.
7
Degranulation of leukocytes in chronic granulomatous disease.慢性肉芽肿病中白细胞的脱颗粒作用。
J Clin Invest. 1969 Jan;48(1):187-92. doi: 10.1172/JCI105967.
8
Effect of thrombin on the radioactive nucleotides of human washed platelets.凝血酶对人洗涤血小板放射性核苷酸的影响。
Biochem J. 1967 Nov;105(2):857-67. doi: 10.1042/bj1050857.
9
Hereditary defect in the platelet release reaction caused by a deficiency in the storage pool of platelet adenine nucleotides.由于血小板腺嘌呤核苷酸储存池缺乏导致的血小板释放反应遗传性缺陷。
Br J Haematol. 1970 Nov;19(5):643-9. doi: 10.1111/j.1365-2141.1970.tb01648.x.
10
Further evidence for a deficient storage pool of adenine nucleotides in platelets from some patients with thrombocytopathia--"storage pool disease".一些血小板病患者（“储存池病”）血小板中腺嘌呤核苷酸储存池缺陷的进一步证据。
Blood. 1972 Feb;39(2):197-209.

人血小板中无机焦磷酸的鉴定及其在凝血酶刺激下的释放。

Identification of inorganic pyrophosphate in human platelets and its release on stimulation with thrombin.

作者信息

Silcox D C, Jacobelli S, McCarty D J

出版信息

J Clin Invest. 1973 Jul;52(7):1595-600. doi: 10.1172/JCI107336.

DOI:10.1172/JCI107336
PMID:4352460
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC302430/
Abstract

Serum inorganic pyrophosphate (PPi) levels were consistently two- to threefold higher than plasma PPi prepared from the same blood. PPi was found in platelets in amounts ranging from 1.4 to 3 nmol/10(8) cells, using three different techniques for quantification. These levels are approximately 800 times higher than the mean PPi concentration in normal plasma and approximate the levels of ADP found in platelets by other workers. About 50% of platelet PPi was specifically released extracellularly after stimulation with thrombin. Timed release experiments showed a pattern of release that resembled that described for ADP and ATP. This pattern was clearly different from that shown by platelet calcium, serotonin, or beta-glucuronidase. Platelet inorganic pyrophosphatase was not released into the supernate in detectable amounts. Platelets from patients with nucleotide storage pool deficiency showed greatly reduced levels of PPi as compared with control. There was no detectable release of PPi into extracellular medium after thrombin addition to a suspension of these platelets.

摘要

血清无机焦磷酸（PPi）水平始终比从同一血液制备的血浆PPi高两到三倍。使用三种不同的定量技术，在血小板中发现的PPi量为1.4至3 nmol/10⁸个细胞。这些水平比正常血浆中的平均PPi浓度高约800倍，接近其他研究人员在血小板中发现的ADP水平。用凝血酶刺激后，约50%的血小板PPi特异性释放到细胞外。定时释放实验显示的释放模式类似于ADP和ATP的释放模式。这种模式明显不同于血小板钙、5-羟色胺或β-葡萄糖醛酸酶的释放模式。血小板无机焦磷酸酶没有以可检测的量释放到上清液中。与对照组相比，核苷酸储存池缺乏症患者的血小板PPi水平大大降低。向这些血小板悬液中加入凝血酶后，未检测到PPi释放到细胞外介质中。