从鸡红细胞核蛋白中逐步去除组蛋白。
The stepwise removal of histones from chicken erythrocyte nucleoprotein.
作者信息
Murray K, Vidali G, Neelin J M
出版信息
Biochem J. 1968 Mar;107(2):207-15. doi: 10.1042/bj1070207.
Abstract
- A fractionation of chicken erythrocyte histones was achieved simultaneously with their extraction from saline-washed nuclei by stepwise titrations to progressively lower pH values. 2. Different acids and dilute buffer solutions of comparable pH behaved similarly in stepwise extractions of histones. 3. The histone preparations so obtained were characterized by their amino acid composition and behaviour on zone electrophoresis in starch gels. 4. The fractionation by titration was quite sharp at appropriate pH ranges, and the histone fraction that is apparently unique to avian erythrocytes was obtained without contamination by other histone fractions. 5. Histones prepared by stepwise titration were fractionated further by cation-exchange and exclusion chromatography. The chromatographic behaviour and amino acid composition of the components permitted comparison with histones prepared by other methods. 6. Histone fraction IIb was resolved into its subfractions IIb(1) and IIb(2) by exclusion chromatography on Bio-Gel P-60. 7. Histone fractions III and IV, previously reported to be absent from chicken erythrocyte nuclei, were found in extracts made at pH1.
摘要
- 通过逐步滴定至逐渐降低的pH值，在从盐水洗涤过的细胞核中提取鸡红细胞组蛋白的同时，实现了对其的分级分离。2. 在组蛋白的逐步提取过程中，不同的酸和pH值相当的稀释缓冲溶液表现相似。3. 如此获得的组蛋白制剂通过其氨基酸组成和在淀粉凝胶区带电泳中的行为来表征。4. 在适当的pH范围内，通过滴定进行的分级分离相当明显，并且获得了显然是鸟类红细胞特有的组蛋白级分，而没有受到其他组蛋白级分的污染。5. 通过逐步滴定制备的组蛋白通过阳离子交换和排阻色谱进一步分级分离。各组分的色谱行为和氨基酸组成允许与通过其他方法制备的组蛋白进行比较。6. 通过在Bio-Gel P-60上进行排阻色谱，组蛋白级分IIb被解析为其亚级分IIb(1)和IIb(2)。7. 先前报道鸡红细胞核中不存在的组蛋白级分III和IV，在pH1条件下制备的提取物中被发现。
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