结合胆酸和放射性标记甘胆酸在大鼠肝脏中的核内与胞质分布
Nuclear and cytosolic distribution of conjugated cholic acid and radiolabelled glycocholic acid in rat liver.
作者信息
Strange R C, Beckett G J, Percy-Robb I W
出版信息
Biochem J. 1979 Jan 15;178(1):71-8. doi: 10.1042/bj1780071.
Abstract
- Normally fed and cholestyramine-treated rats were injected through the superior mesenteric vein with different amounts of radiolabelled glycoholic acid and the appearance of radioactivity in bile was measured. 2. In normally fed rats radioactivity appeared in bile within 30 s of injection and reached a maximum after 2 1/2 min; in the cholestyramine-treated animals the appearance of radioactivity was slower and less of the injected material was excreted into bile. 3. At 10 min after injection, livers were removed and the amounts of radioactive glycoholic acid and endogenous cholic acid conjugates in nuclei and cytosol were determined; most of the bile acid was found in the cytosol, only small amounts being found in nuclei. 4. Nuclear preparations from both normally fed and cholestyramine-fed rats were extracted with KCl (0.4 M) in an attempt to identify a putative bile acid receptor, but no such receptor was found. 5. Regulation of bile acid synthesis does not involve nuclear binding of bile acids.
摘要
- 对正常喂食和用消胆胺处理的大鼠通过肠系膜上静脉注射不同量的放射性标记乙醇酸，并测定胆汁中放射性的出现情况。2. 在正常喂食的大鼠中，注射后30秒内胆汁中出现放射性，并在2分半钟后达到最大值；在用消胆胺处理的动物中，放射性的出现较慢，且注入物质中较少排泄到胆汁中。3. 注射后10分钟，取出肝脏，测定细胞核和细胞质中放射性乙醇酸和内源性胆酸结合物的量；大部分胆汁酸存在于细胞质中，仅在细胞核中发现少量。4. 对正常喂食和用消胆胺喂食的大鼠的细胞核制剂用0.4M氯化钾进行提取，试图鉴定一种假定的胆汁酸受体，但未发现这种受体。5. 胆汁酸合成的调节不涉及胆汁酸的核结合。
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Nuclear and cytosolic distribution of conjugated cholic acid and radiolabelled glycocholic acid in rat liver.结合胆酸和放射性标记甘胆酸在大鼠肝脏中的核内与胞质分布
Biochem J. 1979 Jan 15;178(1):71-8. doi: 10.1042/bj1780071.
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Subcellular distribution of 3-H-estradiol in rat uterus by quantitative autoradiography--a comparison between 3-H-estradiol and 3-H-norethynodrel.通过定量放射自显影术研究大鼠子宫中3-H-雌二醇的亚细胞分布——3-H-雌二醇与3-H-炔诺孕酮的比较
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J Lipid Res. 1970 Sep;11(5):404-11.
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