在复制小鼠肉瘤-白血病病毒的细胞的多核糖体中的病毒特异性信使核糖核酸和新生多肽。
Virus-specific messenger RNA and nascent polypeptides in polyribosomes of cells replicating murine sarcoma-leukemia viruses.
作者信息
Vecchio G, Tsuchida N, Shanmugam G, Green M
出版信息
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2064-8. doi: 10.1073/pnas.70.7.2064.
Abstract
We present evidence that virus-specific RNA is present in polyribosomes of transformed cells replicating the murine sarcoma-leukemia virus complex and that it serves as messenger RNA for the synthesis of viral-coded proteins. Both virus-specific RNA (detected by hybridization with the [(3)H]DNA product of the viral RNA-directed DNA polymerase) and nascent viral polypeptides (measured by precipitation with antiserum to purified virus) were found in membrane-bound and free polyribosomes. Membrane-bound polyribosomes contained a higher content of both virus-specific RNA and nascent viral polypeptides. From 60 to 70% of viral RNA sequences were released from polyribosomes with EDTA, consistent with a function as messenger RNA. Maximum amounts of both virus-specific RNA and nascent viral polypeptides were found in the polyribosome region sedimenting at about 350 S.
摘要
我们提供的证据表明，在复制小鼠肉瘤-白血病病毒复合物的转化细胞的多核糖体中存在病毒特异性RNA，并且它作为信使RNA用于合成病毒编码的蛋白质。在膜结合和游离的多核糖体中均发现了病毒特异性RNA（通过与病毒RNA指导的DNA聚合酶的[(3)H]DNA产物杂交检测）和新生病毒多肽（通过用抗纯化病毒的抗血清沉淀测量）。膜结合的多核糖体中病毒特异性RNA和新生病毒多肽的含量更高。60%至70%的病毒RNA序列可被EDTA从多核糖体中释放出来，这与信使RNA的功能一致。在沉降系数约为350S的多核糖体区域中发现了病毒特异性RNA和新生病毒多肽的最大量。
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