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对肉瘤阳性-白血病阴性转化的3T3细胞产生的非感染性病毒粒子的RNA进行表征。

Characterization of RNA from noninfectious virions produced by sarcoma positive-leukemia negative transformed 3T3 cells.

作者信息

Phillips L A, Hollis V W, Bassin R H, Fischinger P J

出版信息

Proc Natl Acad Sci U S A. 1973 Oct;70(10):3002-6. doi: 10.1073/pnas.70.10.3002.

Abstract

RNA from noninfectious virions produced by two established clonal lines of sarcoma positive-leukemia negative (S+L-)-transformed 3T3 cells has been characterized. RNA from virions or nucleoids of S+L--(C243) cells consisted of three to four sizes: +/-44 S (6%), 28 S (17%), 18 S (38%), and <18 S (39%). 28S virion RNA contained some virus-specific information demonstrable by RNA.DNA hybridization with a DNA probe derived from the RNA-directed DNA polymerase product of murine sarcoma-leukemia virus, while parallel studies indicated that 28S ribosomal RNA from ribosomal subunits of transformed and nontransformed 3T3 cells did not contain virus-specific information. In contrast to the S+L-(C243) virions, RNA from virions or nucleoids of S+L-(D56) cells consisted of five sizes: 80 S (6%), 68 S (8%), 56 S (5%), 28 S (28%), and <28 S (53%). Thermal dissociation studies suggested that this S+L- genome is comprised of 28S RNA subunits. From these studies we postulate that the 28S viral RNA is most probably the monomeric genome of S+L- virions.

摘要

对由两个已建立的肉瘤阳性 - 白血病阴性(S + L - )转化的3T3细胞克隆系产生的非感染性病毒粒子的RNA进行了表征。来自S + L - (C243)细胞的病毒粒子或核仁的RNA由三到四种大小组成:+/- 44S(6%)、28S(17%)、18S(38%)和<18S(39%)。28S病毒粒子RNA包含一些病毒特异性信息,通过与源自鼠肉瘤 - 白血病病毒的RNA指导的DNA聚合酶产物的DNA探针进行RNA - DNA杂交可证明,而平行研究表明,来自转化和未转化的3T3细胞核糖体亚基的28S核糖体RNA不包含病毒特异性信息。与S + L - (C243)病毒粒子相反,来自S + L - (D56)细胞的病毒粒子或核仁的RNA由五种大小组成:80S(6%)、68S(8%)、56S(5%)、28S(28%)和<28S(53%)。热解离研究表明,这种S + L - 基因组由28S RNA亚基组成。从这些研究中我们推测,28S病毒RNA很可能是S + L - 病毒粒子的单体基因组。

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