韭葱（葱属植物韭葱）细胞色素c的氨基酸序列。
The amino acid sequence of cytochrome c from Allium porrum L. (leek).
作者信息
Brown R H, Boulter D
出版信息
Biochem J. 1973 Feb;131(2):247-51. doi: 10.1042/bj1310247.
The amino acid sequence of leek cytochrome c was determined with 0.4mumol of protein. The sequence was deduced solely from a chymotryptic digest. The cytochrome was homologous with other plant cytochromes c of mitochondrial origin. Leek cytochrome c has an N-acetylated ;tail' as compared with mammalian cytochrome c, and two residues of epsilon-N-trimethyl-lysine. Unlike other plant cytochromes c, leek cytochrome c has glutamic acid or glutamine in position 11, leucine in position 20 and alanine in position 51. 4-Hydroxyproline partially substitutes for proline in position 79. Experimental details are given in a supplementary paper that has been deposited as Supplementary Publication SUP 50012, at the National Lending Library for Science and Technology, Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1973) 131, 5.
用0.4微摩尔蛋白质测定了韭菜细胞色素c的氨基酸序列。该序列完全由胰凝乳蛋白酶消化产物推导得出。该细胞色素与其他线粒体来源的植物细胞色素c同源。与哺乳动物细胞色素c相比，韭菜细胞色素c具有N-乙酰化的“尾巴”，以及两个ε-N-三甲基赖氨酸残基。与其他植物细胞色素c不同，韭菜细胞色素c在第11位是谷氨酸或谷氨酰胺，第20位是亮氨酸，第51位是丙氨酸。4-羟基脯氨酸部分替代了第79位的脯氨酸。实验细节见一篇补充论文，该论文已作为补充出版物SUP 50012存放在英国约克郡波士顿温泉市国家科技出借图书馆，地址为LS23 7BQ，可按《生物化学杂志》（1973年）第131卷第5期所示条件从该图书馆获取复印件。
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