烟酰胺腺嘌呤二核苷酸生物合成缺陷的大肠杆菌突变体的交叉喂养
Cross-feeding of Escherichia coli mutants defective in the biosynthesis of nicotinamide adenine dinucleotide.
作者信息
Kerr T J, Tritz G J
出版信息
J Bacteriol. 1973 Sep;115(3):982-6. doi: 10.1128/jb.115.3.982-986.1973.
Abstract
Mutants of Escherichia coli defective in the biosynthesis of nicotinamide adenine dinucleotide (NAD) are able to grow in a Casamino Acids medium lacking NAD and its immediate precursors, nicotinic acid and nicotinamide. This property has allowed the development of a system to measure cross-feeding between a nadA and a nadB mutant. This system provides a means of isolating the intermediate, prequinolinic acid, as well as a biological assay for the compound. The nadB mutant feeds the nadA mutant, indicating that the nadA enzyme occurs first in the pathway and the nadB enzyme second. No cross-feeding was detected between nadA and nadC or between nadB and nadC.
摘要
在烟酰胺腺嘌呤二核苷酸（NAD）生物合成中存在缺陷的大肠杆菌突变体能够在缺乏NAD及其直接前体烟酸和烟酰胺的酪蛋白氨基酸培养基中生长。这一特性使得能够开发出一种系统来测量nadA突变体和nadB突变体之间的交叉喂养。该系统提供了一种分离中间产物前喹啉酸的方法，以及对该化合物的生物学检测方法。nadB突变体为nadA突变体提供营养，这表明nadA酶在该途径中首先出现，nadB酶其次出现。未检测到nadA和nadC之间或nadB和nadC之间的交叉喂养。
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2
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Biochim Biophys Acta. 1967 Jun 13;141(1):199-201. doi: 10.1016/0304-4165(67)90265-6.
3
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4
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5
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