Wolff J, Temple R, Cook G H
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2741-4. doi: 10.1073/pnas.70.10.2741.
Choleragen, the pure protein from cholera toxin, stimulates steroid secretion by Y-1 adrenal tumor cells in culture. The secreted steroids are the same as seen after addition of adrenocorticotropic hormone. Half-maximal stimulation occurs at 15 pM; stimulation is essentially irreversible by washing and partially reversible (for about 1 hr) by antibody, and there is a latent period of about 60 min before stimulation is seen. Stimulation of adenylate cyclase occurs at about 30-fold higher choleragen concentrations. Gangliosides inhibit choleragen stimulation when added before but not after the toxin. Lipopolysaccharides from Escherichia coli, Salmonella typhosa, and Serratia marcescens also stimulate steroid secretion, but are less potent than choleragen.
霍乱毒素中的纯蛋白霍乱原可刺激培养的Y-1肾上腺肿瘤细胞分泌类固醇。分泌的类固醇与添加促肾上腺皮质激素后所见的相同。半最大刺激发生在15皮摩尔;通过洗涤刺激基本不可逆,而通过抗体部分可逆(约1小时),并且在观察到刺激之前有大约60分钟的潜伏期。在大约高30倍的霍乱原浓度下会发生腺苷酸环化酶的刺激。神经节苷脂在毒素之前而非之后添加时可抑制霍乱原刺激。来自大肠杆菌、伤寒沙门氏菌和粘质沙雷氏菌的脂多糖也能刺激类固醇分泌,但效力不如霍乱原。