霍乱毒素对肾上腺肿瘤细胞中类固醇分泌的刺激作用。
Stimulation of steroid secretion in adrenal tumor cells by choleragen.
作者信息
Wolff J, Temple R, Cook G H
出版信息
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2741-4. doi: 10.1073/pnas.70.10.2741.
Abstract
Choleragen, the pure protein from cholera toxin, stimulates steroid secretion by Y-1 adrenal tumor cells in culture. The secreted steroids are the same as seen after addition of adrenocorticotropic hormone. Half-maximal stimulation occurs at 15 pM; stimulation is essentially irreversible by washing and partially reversible (for about 1 hr) by antibody, and there is a latent period of about 60 min before stimulation is seen. Stimulation of adenylate cyclase occurs at about 30-fold higher choleragen concentrations. Gangliosides inhibit choleragen stimulation when added before but not after the toxin. Lipopolysaccharides from Escherichia coli, Salmonella typhosa, and Serratia marcescens also stimulate steroid secretion, but are less potent than choleragen.
摘要
霍乱毒素中的纯蛋白霍乱原可刺激培养的Y-1肾上腺肿瘤细胞分泌类固醇。分泌的类固醇与添加促肾上腺皮质激素后所见的相同。半最大刺激发生在15皮摩尔；通过洗涤刺激基本不可逆，而通过抗体部分可逆（约1小时），并且在观察到刺激之前有大约60分钟的潜伏期。在大约高30倍的霍乱原浓度下会发生腺苷酸环化酶的刺激。神经节苷脂在毒素之前而非之后添加时可抑制霍乱原刺激。来自大肠杆菌、伤寒沙门氏菌和粘质沙雷氏菌的脂多糖也能刺激类固醇分泌，但效力不如霍乱原。
相似文献
1
Stimulation of steroid secretion in adrenal tumor cells by choleragen.霍乱毒素对肾上腺肿瘤细胞中类固醇分泌的刺激作用。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2741-4. doi: 10.1073/pnas.70.10.2741.
2
Choleragen stimulates steroidogenesis and adenylate cyclase in cells lacking functional hormone receptors.霍乱毒素可刺激缺乏功能性激素受体的细胞中的类固醇生成和腺苷酸环化酶。
Biochim Biophys Acta. 1975 Dec 1;413(2):283-90. doi: 10.1016/0005-2736(75)90113-3.
3
Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin. Relations to the mode of activation by hormones.霍乱弧菌肠毒素激活腺苷酸环化酶的机制。与激素激活方式的关系。
J Membr Biol. 1975 Nov 7;24(2):107-29. doi: 10.1007/BF01868618.
4
Endotoxic lipopolysaccharides stimulate steroidogenesis and adenylate cyclase in adrenal tumor cells.内毒素脂多糖刺激肾上腺肿瘤细胞中的类固醇生成和腺苷酸环化酶。
Biochim Biophys Acta. 1975 Dec 1;413(2):291-7. doi: 10.1016/0005-2736(75)90114-5.
5
The effect of cholera enterotoxin on steroidogenesis in cultured adrenal tumor cells.霍乱肠毒素对培养的肾上腺肿瘤细胞类固醇生成的影响。
J Infect Dis. 1974 Jun;129(6):690-5. doi: 10.1093/infdis/129.6.690.
6
Mechanism of activation of adenylate cyclase by cholera toxin.霍乱毒素激活腺苷酸环化酶的机制。
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3438-42. doi: 10.1073/pnas.72.9.3438.
7
Interaction of cholera enterotoxin with cultured adrenal tumor cells.霍乱肠毒素与培养的肾上腺肿瘤细胞的相互作用。
Infect Immun. 1975 Oct;12(4):768-71. doi: 10.1128/iai.12.4.768-771.1975.
8
Adrenal cells in tissue culture the effects of choleragen and ACTH on steroid and cyclic-AMP metabolism.组织培养中的肾上腺细胞：霍乱毒素和促肾上腺皮质激素对类固醇及环磷酸腺苷代谢的影响。
Endocr Res Commun. 1975;2(1):65-86. doi: 10.1080/07435807509053839.
9
Mechanism of action of cholera toxin: studies on the lag period.霍乱毒素的作用机制：关于延迟期的研究
J Membr Biol. 1980;54(1):61-72. doi: 10.1007/BF01875377.
10
Escherichia coli enterotoxin-induced steroidogenesis in cultured adrenal tumor cells.大肠杆菌肠毒素诱导培养的肾上腺肿瘤细胞中的类固醇生成。
Infect Immun. 1974 Jul;10(1):146-51. doi: 10.1128/iai.10.1.146-151.1974.
引用本文的文献
1
Cell junction and cycle AMP: III. Promotion of junctional membrane permeability and junctional membrane particles in a junction-deficient cell type.细胞连接与环磷酸腺苷：III. 在一种缺乏连接的细胞类型中促进连接膜通透性和连接膜颗粒
J Membr Biol. 1981;63(1-2):133-46. doi: 10.1007/BF01969454.
2
Mechanism of action of choleragen and E. coli heat-labile enterotoxin: activation of adenylate cyclase by ADP-ribosylation.霍乱毒素和大肠杆菌不耐热肠毒素的作用机制：通过ADP-核糖基化激活腺苷酸环化酶。
Mol Cell Biochem. 1981 Jul 7;37(2):75-90. doi: 10.1007/BF02354931.
3
5'-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells.5'-鸟苷酰亚胺二磷酸，一种真核细胞中腺苷酸环化酶系统的强效激活剂。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3087-90. doi: 10.1073/pnas.71.8.3087.
4
Cholera toxin and cell growth: role of membrane gangliosides.霍乱毒素与细胞生长：膜神经节苷脂的作用
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4224-8. doi: 10.1073/pnas.71.10.4224.
5
Adenosine 3',5'-cyclic monophosphate and adrenal tumor cell morphology.3',5'-环磷酸腺苷与肾上腺肿瘤细胞形态
In Vitro. 1974 Nov-Dec;10(5-6):268-73. doi: 10.1007/BF02615307.
6
Effect of cholera toxin on insulin release in monolayer cultures of the endocrine pancreas.霍乱毒素对内分泌胰腺单层培养物中胰岛素释放的影响。
Diabetologia. 1974 Dec;10(6):783-7. doi: 10.1007/BF01219541.
7
The pancreatic beta-cell recognition of insulin secretagogues: does cyclic AMP mediate the effect of glucose?胰腺β细胞对胰岛素促分泌剂的识别：环磷酸腺苷是否介导葡萄糖的作用？
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3405-9. doi: 10.1073/pnas.71.9.3405.
8
Escherichia coli enterotoxin-induced steroidogenesis in cultured adrenal tumor cells.大肠杆菌肠毒素诱导培养的肾上腺肿瘤细胞中的类固醇生成。
Infect Immun. 1974 Jul;10(1):146-51. doi: 10.1128/iai.10.1.146-151.1974.
9
Cholera toxin mimics melanocyte stimulating hormone in inducing differentiation in melanoma cells.霍乱毒素在诱导黑色素瘤细胞分化方面模拟促黑素细胞激素。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2500-4. doi: 10.1073/pnas.71.6.2500.
10
Mobility of cholera toxin receptors on rat lymphocyte membranes.霍乱毒素受体在大鼠淋巴细胞膜上的流动性
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3844-8. doi: 10.1073/pnas.72.10.3844.
本文引用的文献
1
Monospecific equine antiserum against cholera exo-enterotoxin.马抗霍乱肠毒素单克隆抗体血清。
Infect Immun. 1970 Dec;2(6):691-7. doi: 10.1128/iai.2.6.691-697.1970.
2
Hormone-producing cultures of adrenal and pituitary tumor origin.源自肾上腺和垂体肿瘤的激素分泌培养物。
Proc Natl Acad Sci U S A. 1962 Jul 15;48(7):1184-90. doi: 10.1073/pnas.48.7.1184.
3
Kinetics and dose-response characteristics of adenosine 3',5'-monophosphate production by isolated rat adrenal cells stimulated with adrenocorticotrophic hormone.促肾上腺皮质激素刺激的离体大鼠肾上腺细胞产生3',5'-单磷酸腺苷的动力学和剂量反应特征
FEBS Lett. 1972 Jul 1;23(3):345-348. doi: 10.1016/0014-5793(72)80312-0.
4
Time of onset of action of cholera toxin in dog and rabbit.霍乱毒素在犬和兔体内的起效时间。
Gastroenterology. 1969 Jul;57(1):5-8.
5
Effects of vasopressin, theophylline and cyclic adenosine monophosphate on short-circuit current across isolated rabbit ileal mucosa.血管加压素、茶碱和环磷酸腺苷对离体兔回肠黏膜短路电流的影响。
Nature. 1968 Feb 3;217(5127):469-71. doi: 10.1038/217469a0.
6
7
Deactivation of cholera toxin by ganglioside.神经节苷脂对霍乱毒素的失活作用
J Infect Dis. 1971 Oct;124(4):415-8. doi: 10.1093/infdis/124.4.415.
8
Intestinal adenyl-cyclase activity in canine cholera: correlation with fluid accumulation.犬霍乱时肠道腺苷酸环化酶活性：与液体蓄积的相关性。
J Infect Dis. 1972 Apr;125(4):377-81. doi: 10.1093/infdis/125.4.377.
9
Chemical and physical properties of cholera exo-enterotoxin (choleragen) and its spontaneously formed toxoid (choleragenoid).霍乱外毒素（霍乱原）及其自发形成的类毒素（类霍乱原）的化学和物理性质。
Biochim Biophys Acta. 1972 Jan 26;257(1):158-66. doi: 10.1016/0005-2795(72)90265-6.
10
Selective stimulation of epinephrine-responsive adenyl cyclase in mice by endotoxin.内毒素对小鼠肾上腺素反应性腺苷酸环化酶的选择性刺激作用。
Proc Soc Exp Biol Med. 1971 Dec;138(3):773-5. doi: 10.3181/00379727-138-35986.
Zotero 插件