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A mutant of 3T3 cells with cyclic AMP metabolism sensitive to temperature change.

作者信息

Willingham M C, Carchman R A, Pastan I H

出版信息

Proc Natl Acad Sci U S A. 1973 Oct;70(10):2906-10. doi: 10.1073/pnas.70.10.2906.

DOI:10.1073/pnas.70.10.2906
PMID:4355372
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427136/
Abstract

A mutant line of 3T3 (mouse fibroblast) cells with temperature-sensitive cyclic AMP (cAMP) metabolism was isolated by selecting for low substratum adhesiveness after a change in temperature. Although the mutant is identical in behavior to the parent cell at constant temperature, a rise or fall in temperature causes a fall in intracellular cAMP levels within seconds, followed by loss of adherence to the culture dish and retraction of cell processes. The mechanism of this fall in cAMP level is at least in part excretion into the medium. The decrease in adhesiveness and retraction of processes can be blocked by analogues of cAMP or agents that elevate intracellular cAMP. The properties of this mutant imply that cAMP is a direct regulator of cell shape and adhesiveness.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/cdeb05504dfa/pnas00137-0198-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/e9d35702e60f/pnas00137-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/ef66f634da34/pnas00137-0197-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/a555e4012942/pnas00137-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/cdeb05504dfa/pnas00137-0198-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/e9d35702e60f/pnas00137-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/ef66f634da34/pnas00137-0197-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/a555e4012942/pnas00137-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed8/427136/cdeb05504dfa/pnas00137-0198-b.jpg

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引用本文的文献

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本文引用的文献

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Altered nucleic acid metabolism in human cell cultures infected with mycoplasma.感染支原体的人类细胞培养物中核酸代谢的改变。
Proc Natl Acad Sci U S A. 1968 Jun;60(2):583-9. doi: 10.1073/pnas.60.2.583.
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Induction of the lactose transport system in a lipid-synthesis-defective mutant of Escherichia coli.大肠杆菌脂质合成缺陷型突变体中乳糖转运系统的诱导。
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The effect of membrane lipid unsaturation on glycoside transport.膜脂不饱和度对糖苷转运的影响。
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Cyclic AMP mediates the concanavalin A agglutinability of mouse fibroblasts.环磷酸腺苷介导小鼠成纤维细胞的伴刀豆球蛋白A凝集性。
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Mechanisms of membrane damage for CHO cells heated in suspension.悬浮培养的中国仓鼠卵巢细胞受热时的膜损伤机制。
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Cyclic AMP, the microtubule-microfilament system, and cancer.环磷酸腺苷、微管-微丝系统与癌症。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4491-5. doi: 10.1073/pnas.74.10.4491.
7
Changes in surface properties of normal and transformed cells caused by tunicamycin, an inhibitor of protein glycosylation.衣霉素（一种蛋白质糖基化抑制剂）引起的正常细胞和转化细胞表面性质的变化。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3433-7. doi: 10.1073/pnas.74.8.3433.
8
Cell to substratum adhesion-promoting activity released by normal and virus-transformed cells in culture.培养中的正常细胞和病毒转化细胞释放的促进细胞与基质黏附的活性。
J Cell Biol. 1976 Sep;70(3):634-47. doi: 10.1083/jcb.70.3.634.
9
Mutants of Balb/c 3T3 fibroblasts defective in adhesiveness to substratum: evidence for alteration in cell surface proteins.对底物黏附性有缺陷的Balb/c 3T3成纤维细胞突变体：细胞表面蛋白改变的证据。
Proc Natl Acad Sci U S A. 1976 Feb;73(2):544-8. doi: 10.1073/pnas.73.2.544.
10
Antagonism by dibutyryl adenosine cyclic 3',5'-monophosphate and testololactone of concanavalin A capping.二丁酰腺苷环 3',5'-单磷酸和睾酮内酯对刀豆球蛋白A帽化的拮抗作用
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4
Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase.缺乏β-羟基癸酰硫酯脱水酶的大肠杆菌脂肪酸突变体。
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5
Transformation of chick-embryo fibroblasts by wild-type and temperature-sensitive Rous sarcoma virus alters adenylate cyclase activity.野生型和温度敏感型劳氏肉瘤病毒对鸡胚成纤维细胞的转化会改变腺苷酸环化酶的活性。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1055-9. doi: 10.1073/pnas.70.4.1055.
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Cyclic AMP and growth of fibroblasts: effect of environmental pH.环磷酸腺苷与成纤维细胞生长：环境pH值的影响
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The effect of dibutyryl adenosine 3':5'-cyclic monophosphate on the synthesis of glycolipids by normal and transformed Nil cells.
Arch Biochem Biophys. 1973 Jan;154(1):407-14. doi: 10.1016/0003-9861(73)90073-8.
8
Effects of dibutyryl cyclic adenosine phosphate plus theophylline on murine sarcoma virus transformed non-producer cells.磷酸二丁酰环磷腺苷加茶碱对鼠肉瘤病毒转化的非产生细胞的作用
Proc Soc Exp Biol Med. 1972 Dec;141(3):1044-50. doi: 10.3181/00379727-141-36930.
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Control of DNA synthesis and mitosis in 3T3 cells by cyclic AMP.环磷酸腺苷对3T3细胞中DNA合成和有丝分裂的调控
Biochem Biophys Res Commun. 1972 Aug 21;48(4):743-8. doi: 10.1016/0006-291x(72)90669-9.
10
Regulation of cell growth by cyclic adenosine 3',5'-monophosphate. Effect of cell density and agents which alter cell growth on cyclic adenosine 3',5'-monophosphate levels in fibroblasts.3',5'-环磷酸腺苷对细胞生长的调节。细胞密度及改变细胞生长的因子对成纤维细胞中3',5'-环磷酸腺苷水平的影响。
J Biol Chem. 1972 Nov 10;247(21):7082-7.