Kudelin B K, Kaminskiĭ Iu L, Ivanova I F, Gavrilin S S
Biokhimiia. 1979 Feb;44(2):368-71.
New solvent systems--dioxane--isopropanol--water--ammonium (4:2:4:1) and dioxane--water--ammonium (6:4:1)--for rapid qualitative and quantitative estimation of nucleosides, nucleoside-5'-mono-, di- and triphosphates by thin layer chromatography is Silufol UV-254 are proposed. The Rf values for 40 ribo- and deoxyribonucleotides of these systems are given.
提出了新的溶剂体系——二氧六环——异丙醇——水——铵(4:2:4:1)和二氧六环——水——铵(6:4:1),用于通过硅胶UV - 254薄层色谱法对核苷、核苷5'-单磷酸、二磷酸和三磷酸进行快速定性和定量测定。给出了这些体系中40种核糖核苷酸和脱氧核糖核苷酸的比移值。