干扰素治疗的抗病毒效果与未感染的L细胞中体外mRNA翻译抑制之间的相关性。
Correlation between the antiviral effect of interferon treatment and the inhibition of in vitro mRNA translation in noninfected L cells.
作者信息
Falcoff E, Falcoff R, Lebleu B, Revel M
出版信息
J Virol. 1973 Sep;12(3):421-30. doi: 10.1128/JVI.12.3.421-430.1973.
Abstract
When noninfected L-cell suspension cultures are treated with interferon (specific activities superior to 10(6) reference units per mg of protein), the cell-free cytoplasmic extracts obtained are inactive for the translation of exogenous natural mRNAs. The dose-response curve shows that comparable amounts of interferon are required to produce a 50% reduction of Mengo virus multiplication in vivo and Mengo RNA translation in vitro. With higher doses of interferon, Mengo RNA translation is completely abolished, while poly U translation and endogenous protein synthesis are only slightly affected. The inactivation of Mengo RNA translation is reversible; after removal of interferon, normal translation activity is regained together with the ability to support Mengo virus multiplication. Fractionation of the cell-free extracts shows that the effect is localized in the fraction which can be washed off the ribosomes by high salt. These results establish that interferon induces a block in genetic translation in noninfected L cells.
摘要
当用干扰素（比活性高于每毫克蛋白质10⁶参考单位）处理未感染的L细胞悬浮培养物时，所获得的无细胞胞质提取物对外源天然mRNA的翻译无活性。剂量反应曲线表明，在体内使门戈病毒增殖减少50%和在体外使门戈RNA翻译减少50%需要相当量的干扰素。使用更高剂量的干扰素时，门戈RNA翻译完全被抑制，而聚尿苷酸翻译和内源性蛋白质合成仅受到轻微影响。门戈RNA翻译的失活是可逆的；去除干扰素后，正常翻译活性以及支持门戈病毒增殖的能力得以恢复。对无细胞提取物进行分级分离表明，这种作用局限于能用高盐从核糖体上洗脱下来的级分中。这些结果证实，干扰素在未感染的L细胞中诱导了基因翻译的阻断。
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