呼吸驱动的酵母线粒体质子转运，其氧化磷酸化效率各异。
Respiration-driven proton translocation by yeast mitochondria with differing efficiencies of oxidative phosphorylation.
作者信息
Downie J A, Garland P B
出版信息
Biochem J. 1973 Aug;134(4):1045-9. doi: 10.1042/bj1341045.
Abstract
Measurements were made of the stoicheiometry of proton translocation coupled to respiration in mitochondria from Candida utilis where the number of functional energy-conservation sites between intramitochondrial NADH and oxygen was one in a mutant with a novel oxidase (Downie & Garland, 1972), two in sulphate-deficient cells (Haddock & Garland, 1971) or three in glycerol-limited cells (Light & Garland, 1971). The stoicheiometries of protons translocated per atom of oxygen utilized (i.e. -->H(+)/2e(-) ratio; Mitchell, 1966) were close to 2.0, 4.0 and 6.0 respectively. Thus by using the same substrate (intramitochondrial NADH) and oxygen throughout, the -->H(+)/2e(-) ratio is shown to be 2.0 per energy-conservation site when the number of such sites is varied from one to three.
摘要
对产朊假丝酵母线粒体中与呼吸偶联的质子转运化学计量进行了测量，在一种具有新型氧化酶的突变体中，线粒体内NADH与氧气之间的功能能量保守位点数量为1个（唐尼和加兰，1972年），在硫酸盐缺乏的细胞中为2个（哈多克和加兰，1971年），在甘油限制的细胞中为3个（莱特和加兰，1971年）。每利用一个氧原子转运的质子化学计量（即H⁺/2e⁻比率；米切尔，1966年）分别接近2.0、4.0和6.0。因此，通过始终使用相同的底物（线粒体内NADH）和氧气，当这种位点的数量从1个变化到3个时，每个能量保守位点的H⁺/2e⁻比率显示为2.0。
相似文献
1
Respiration-driven proton translocation by yeast mitochondria with differing efficiencies of oxidative phosphorylation.呼吸驱动的酵母线粒体质子转运，其氧化磷酸化效率各异。
Biochem J. 1973 Aug;134(4):1045-9. doi: 10.1042/bj1341045.
2
Proton translocation coupled to quinone reduction by reduced nicotinamide--adenine dinucleotide in rat liver and ox heart mitochondria.大鼠肝脏和牛心脏线粒体中质子转运与还原型烟酰胺腺嘌呤二核苷酸介导的醌还原相偶联。
Biochem J. 1972 Dec;130(4):1029-44. doi: 10.1042/bj1301029.
3
The microbial metabolism of Cl compounds. The stoicheiometry of respiration-driven proton translocation in Pseudomonas AM1 and in a mutant lacking cytochrome c.氯化合物的微生物代谢。假单胞菌AM1及缺乏细胞色素c的突变体中呼吸驱动质子转运的化学计量学。
Biochem J. 1978 Mar 15;170(3):561-7. doi: 10.1042/bj1700561.
4
Respiration-driven proton translocation in Escherichia coli.大肠杆菌中呼吸驱动的质子转运
Biochem J. 1973 Sep;136(1):217-20. doi: 10.1042/bj1360217.
5
Translocation of protons and potassium ions across the mitochondrial membrane of respiring and respiration-deficient yeasts.质子和钾离子跨呼吸型和呼吸缺陷型酵母线粒体膜的转运
Biochim Biophys Acta. 1972 Jan 21;256(1):55-65. doi: 10.1016/0005-2728(72)90162-4.
6
The H+/e- stoicheiometry of respiration-linked proton translocation in the cytochrome system of mitochondria.线粒体细胞色素系统中呼吸链质子转运的H⁺/e⁻化学计量关系。
Biochem J. 1980 Oct 15;192(1):203-18. doi: 10.1042/bj1920203.
7
The mechanism of transmembrane delta muH+ generation in mitochondria by cytochrome c oxidase.细胞色素c氧化酶在线粒体中产生跨膜质子动力势（delta muH+）的机制。
Biochem J. 1979 Jul 15;182(1):133-47. doi: 10.1042/bj1820133.
8
Respiration-linked proton translocation in the obligate methylotroph Methylophilus methylotrophus.专性甲基营养菌嗜甲基甲基ophilus中的呼吸相关质子转运。
Biochem J. 1981 Mar 15;194(3):915-24. doi: 10.1042/bj1940915.
9
Inhibition by butylmalonate of proton influx in nonphosphorylating mitochondria.丁基丙二酸对非磷酸化线粒体中质子内流的抑制作用。
Arch Biochem Biophys. 1998 Jul 1;355(1):93-100. doi: 10.1006/abbi.1998.0709.
10
An oligomycin-resistant adenosine triphosphatase and its effects on cellular growth, mitochondrial oxidative phosphorylation and respiratory proton translocation in Saccharomyces cerevisiae.一种抗寡霉素的三磷酸腺苷酶及其对酿酒酵母细胞生长、线粒体氧化磷酸化和呼吸质子转运的影响。
Biochem J. 1977 Jan 15;162(1):51-9. doi: 10.1042/bj1620051.
引用本文的文献
1
On the enzymic mechanism of oxidative phosphorylation.论氧化磷酸化的酶促机制。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1064-8. doi: 10.1073/pnas.79.4.1064.
2
The mechanism of proton translocation driven by the respiratory nitrate reductase complex of Escherichia coli.大肠杆菌呼吸硝酸盐还原酶复合物驱动质子转运的机制。
Biochem J. 1980 Jul 15;190(1):79-94. doi: 10.1042/bj1900079.
3
An antimycin A- and cyanide-resistant variant of Candida utilis arising during copper-limited growth.在铜限制生长过程中产生的解脂假丝酵母抗抗霉素A和氰化物变体。
Biochem J. 1973 Aug;134(4):1051-61. doi: 10.1042/bj1341051.
4
Respiration-driven proton translocation in Escherichia coli.大肠杆菌中呼吸驱动的质子转运
Biochem J. 1973 Sep;136(1):217-20. doi: 10.1042/bj1360217.
5
Determination of the efficiency of oxidative phosphorylation in continuous cultures of Aerobacter aerogenes.产气气杆菌连续培养中氧化磷酸化效率的测定。
Arch Microbiol. 1975 Mar 10;102(3):187-92. doi: 10.1007/BF00428367.
6
Stoichiometric relationship between energy-dependent proton ejection and electron transport in mitochondria.线粒体中能量依赖型质子排出与电子传递之间的化学计量关系。
Proc Natl Acad Sci U S A. 1976 Feb;73(2):437-41. doi: 10.1073/pnas.73.2.437.
7
Energy conservation during aerobic growth in Paracoccus denitrificans.反硝化副球菌有氧生长过程中的能量守恒。
Arch Microbiol. 1977 Feb 4;112(1):25-34. doi: 10.1007/BF00446650.
8
Non electrogenic function of the mitochondrial, alternative, cyanide-insensitive respiration in the yeast Saccharomycopsis lipolytica.解脂耶氏酵母中线粒体交替途径氰化物不敏感呼吸的非生电功能
Arch Microbiol. 1978 Mar;116(3):297-302. doi: 10.1007/BF00417855.
9
Effects of sulphate-limited growth in continuous culture on the electron-transport chain and energy conservation in Escherichia coli K12.连续培养中硫酸盐限制生长对大肠杆菌K12电子传递链和能量守恒的影响。
Biochem J. 1975 Dec;152(3):537-46. doi: 10.1042/bj1520537.
10
Growth yields, polysaccharide production and energy conservation in chemostat cultures of Rhizobium trifolii.三叶草根瘤菌恒化器培养中的生长产量、多糖产生及能量守恒
Antonie Van Leeuwenhoek. 1979;45(3):401-15. doi: 10.1007/BF00443279.
本文引用的文献
1
Respiration-driven proton translocation in rat liver mitochondria.呼吸驱动的大鼠肝线粒体质子转运
Biochem J. 1967 Dec;105(3):1147-62. doi: 10.1042/bj1051147.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
The respiratory chain and oxidative phosphorylation.呼吸链与氧化磷酸化。
Adv Enzymol Relat Subj Biochem. 1956;17:65-134. doi: 10.1002/9780470122624.ch2.
4
Mitochondrial effects of copper-limited growth of Torulopsis utilis.
FEBS Lett. 1972 Jan 1;19(4):319-322. doi: 10.1016/0014-5793(72)80070-x.
5
Biochemical and genetic classification of riboflavine deficient mutants of Saccharomyces cerevisiae.酿酒酵母核黄素缺陷型突变体的生化与遗传分类
Mol Gen Genet. 1969;105(4):306-13. doi: 10.1007/BF00277585.
6
Studies of oxidation phosphorylation in Saccharomyces cerevisiae and Saccharomyces carlsbergensis.酿酒酵母和卡尔斯伯酵母氧化磷酸化的研究。
Biochim Biophys Acta. 1973 Jan 18;292(1):88-91. doi: 10.1016/0005-2728(73)90253-3.
7
Translocation of protons and potassium ions across the mitochondrial membrane of respiring and respiration-deficient yeasts.质子和钾离子跨呼吸型和呼吸缺陷型酵母线粒体膜的转运
Biochim Biophys Acta. 1972 Jan 21;256(1):55-65. doi: 10.1016/0005-2728(72)90162-4.
8
Effect of sulphate-limited growth on mitochondrial electron transfer and energy conservation between reduced nicotinamide-adenine dinucleotide and the cytochromes in Torulopsis utilis.硫酸盐限制生长对产朊假丝酵母中还原型烟酰胺腺嘌呤二核苷酸与细胞色素之间线粒体电子传递和能量守恒的影响。
Biochem J. 1971 Aug;124(1):155-70. doi: 10.1042/bj1240155.
9
An antimycin A- and cyanide-resistant variant of Candida utilis arising during copper-limited growth.在铜限制生长过程中产生的解脂假丝酵母抗抗霉素A和氰化物变体。
Biochem J. 1973 Aug;134(4):1051-61. doi: 10.1042/bj1341051.
10
A comparison of mitochondria from Torulopsis utilis grown in continuous culture with glycerol, iron, ammonium, magnesium or phosphate as the growth-limiting nutrient.对在连续培养中以甘油、铁、铵、镁或磷酸盐作为生长限制营养物培养的产朊假丝酵母的线粒体进行比较。
Biochem J. 1971 Aug;124(1):123-34. doi: 10.1042/bj1240123.
Zotero 插件