Jenkins N, Taylor M W, Raff R A
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3287-91. doi: 10.1073/pnas.70.12.3287.
A cell-free protein-synthesizing system prepared from mouse sarcoma 180 was characterized by use of RNA from mengo virus and sea urchin egg. In the presence of exogenous mammalian transfer RNA, total sea urchin egg RNA and mengo RNA direct incorporation of [(3)H]leucine into acid-insoluble material. The system is extremely efficient in that a stimulation of 100-times over background can be obtained. Studies with formylmethionyl-transfer RNA, as well as with inhibitors of initiation, indicate that multiple initiation occurs; further, 85-90% of all chains made in vitro are subsequently released from ribosomes. An average translation time of 3.5 min was determined with messenger RNA of sea urchin egg, and product analysis indicates that high-molecular-weight products (greater than 50,000 molecular weight) are being made in vitro. Sequences of sea urchin egg RNA containing poly(A) act as messenger RNA.
利用来自门戈病毒和海胆卵的RNA，对从小鼠肉瘤180制备的无细胞蛋白质合成系统进行了表征。在外源哺乳动物转移RNA存在的情况下，总的海胆卵RNA和门戈RNA可将[³H]亮氨酸直接掺入酸不溶性物质中。该系统效率极高，因为可以获得比背景高100倍的刺激。对甲酰甲硫氨酰转移RNA以及起始抑制剂的研究表明发生了多次起始；此外，体外合成的所有链中85 - 90%随后从核糖体上释放。用海胆卵信使RNA测定的平均翻译时间为3.5分钟，产物分析表明体外正在合成高分子量产物（分子量大于50,000）。含有多聚(A)的海胆卵RNA序列充当信使RNA。
