海胆卵母源信使核糖核酸的无细胞翻译
Cell-free translation of maternal messenger RNA from sea urchin eggs.
作者信息
Gross K W, Jacobs-Lorena M, Baglioni C, Gross P R
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2614-8. doi: 10.1073/pnas.70.9.2614.
Abstract
The template activity of RNA extracted from unfertilized sea urchin eggs was demonstrated in a cell-free translation system, and, for the first time, specific proteins were identified among the products. All messenger RNA activity is recovered, under the conditions used, from the postribosomal supernatant. Histones, among many other proteins, were identified specifically as products of this "maternal" messenger RNA.
摘要
在无细胞翻译系统中证实了从未受精的海胆卵中提取的RNA的模板活性，并且首次在产物中鉴定出了特定的蛋白质。在所使用的条件下，所有信使RNA活性都从核糖体后上清液中恢复。在许多其他蛋白质中，组蛋白被明确鉴定为这种“母体”信使RNA的产物。
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