人红细胞完整空影中3':5'-环磷酸腺苷受体的特异性光亲和标记
Specific photoaffinity labeling of the adenosine 3':5'-cyclic monophosphate receptor in intact ghosts from human erythrocytes.
作者信息
Guthrow C E, Rasmussen H, Brunswick D J, Cooperman B S
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3344-6. doi: 10.1073/pnas.70.12.3344.
Abstract
[(3)H]N(6)-(Ethyl 2-diazomalonyl)-adenosine 3':5'-cyclic monophosphate is incorporated into intact ghosts from human erythrocytes on photolysis at 253.7 nm. Incorporation is blocked in the presence of adenosine 3':5'-cyclic monophosphate (cyclic AMP) and does not occur in the absence of irradiation. Sodium dodecyl sulfate disc gel electrophoresis of solubilized ghosts shows that one protein is labeled. The position of this protein on the gel corresponds exactly to that previously found. [J. Biol. Chem. 247, 8145 (1972)] for the endogeneous protein substrate of the endogenous, cyclic AMP-dependent, protein kinase.
摘要
在253.7nm光解时，[（3）H]N（6）-（乙基2-重氮丙二酰基）-腺苷3':5'-环一磷酸被掺入人红细胞的完整细胞膜空壳中。在腺苷3':5'-环一磷酸（环磷酸腺苷）存在的情况下，掺入被阻断，并且在没有照射的情况下不会发生。对溶解的细胞膜空壳进行十二烷基硫酸钠圆盘凝胶电泳显示有一种蛋白质被标记。该蛋白质在凝胶上的位置与先前发现的完全一致。[《生物化学杂志》247, 8145（1972）]中内源性环磷酸腺苷依赖性蛋白激酶的内源性蛋白质底物的位置。
相似文献
1
Specific photoaffinity labeling of the adenosine 3':5'-cyclic monophosphate receptor in intact ghosts from human erythrocytes.人红细胞完整空影中3':5'-环磷酸腺苷受体的特异性光亲和标记
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3344-6. doi: 10.1073/pnas.70.12.3344.
2
Photo-affinity labels for adenosine 3':5'-cyclic monophosphate.3':5'-环磷酸腺苷的光亲和标记物。
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1801-4. doi: 10.1073/pnas.68.8.1801.
3
Adenosine 3':5'-monophosphate-regulated phosphorylation of erythrocyte membrane proteins. Separation of membrane-associated cyclic adenosine 3':5'-monophosphate-dependent protein kinase from its endogenous substrates.腺苷3':5'-单磷酸调节的红细胞膜蛋白磷酸化。膜相关的环腺苷3':5'-单磷酸依赖性蛋白激酶与其内源性底物的分离。
J Biol Chem. 1975 Dec 10;250(23):9044-52.
4
On the photoaffinity labeling of rabbit muscle phosphofructokinase with O2'-(ethyl-2-diazomalonyl)adenosine 3':5'-cyclic monophosphate.用O2'-(乙基-2-重氮丙二酰基)腺苷3':5'-环一磷酸对兔肌肉磷酸果糖激酶进行光亲和标记
Biochemistry. 1973 Oct 9;12(21):4079-84. doi: 10.1021/bi00745a009.
5
Evidence for the participation of cytosolic protein kinases in membrane phosphorylation in intact erythrocytes.胞质蛋白激酶参与完整红细胞膜磷酸化作用的证据。
Eur J Biochem. 1978 Jan 16;82(2):333-7. doi: 10.1111/j.1432-1033.1978.tb12027.x.
6
Studies on protein kinase activity and the binding of adenosine 3'5-monophosphate by membranes of hereditary spherocytosis erythrocytes.遗传性球形红细胞增多症红细胞膜的蛋白激酶活性及3',5'-环磷酸腺苷结合的研究。
Biochem Biophys Res Commun. 1975 Oct 6;66(3):1078-86. doi: 10.1016/0006-291x(75)90750-0.
7
Photoaffinity labeling of a protein kinase from bovine brain with 8-azidoadenosine 3',5'-monophosphate.用8-叠氮腺苷3',5'-单磷酸对牛脑蛋白激酶进行光亲和标记。
Biochemistry. 1975 Aug 26;14(17):3858-62. doi: 10.1021/bi00688a019.
8
Purification and characterization of adenosine-adenosine cyclic 3',5'-monophosphate binding protein factors from rabbit erythrocytes.兔红细胞中腺苷-3',5'-环磷酸腺苷结合蛋白因子的纯化与特性分析
Biochemistry. 1974 Dec 3;13(25):5220-6. doi: 10.1021/bi00722a027.
9
Human erythrocyte proteins associated with adenosine 3',5'-cyclic monophosphate action.与3',5'-环磷酸腺苷作用相关的人红细胞蛋白。
Enzyme. 1978;23(2):73-83. doi: 10.1159/000458555.
10
Photoaffinity labeling of three renal cyclic 3',5'-adenosine monophosphate-binding proteins.三种肾环磷酸腺苷结合蛋白的光亲和标记
Biochim Biophys Acta. 1979 Nov 1;587(4):522-39. doi: 10.1016/0304-4165(79)90006-0.
引用本文的文献
1
The synthesis and biological properties of 8-azido-N (6)-benzyladenine, a potential photoaffinity reagent for cytokinin.8-叠氮-N（6）-苄基腺嘌呤的合成及生物性质，一种潜在的细胞分裂素光亲和试剂。
Planta. 1977 Jan;137(2):91-6. doi: 10.1007/BF00387544.
2
Ultraviolet light-induced labeling by noncompetitive blockers of the acetylcholine receptor from Torpedo marmorata.用非竞争性阻滞剂对斑纹电鳐乙酰胆碱受体进行紫外线诱导标记。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3925-9. doi: 10.1073/pnas.78.6.3925.
3
The organization of proteins in the human red blood cell membrane. A review.人类红细胞膜中蛋白质的组织。综述。
J Cell Biol. 1974 Jul;62(1):1-19. doi: 10.1083/jcb.62.1.1.
4
An examination of the utility of photogenerated reagents by using alpha-chymotrypsin.通过使用α-胰凝乳蛋白酶对光生试剂的效用进行考察。
Biochem J. 1974 Dec;143(3):663-8. doi: 10.1042/bj1430663.
5
Interactions of a photo-affinity ATP analog with cation-stimulated adenosine triphosphatases of human red cell membranes.一种光亲和性ATP类似物与人红细胞膜阳离子刺激的三磷酸腺苷酶的相互作用。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3367-71. doi: 10.1073/pnas.71.9.3367.
6
Photoaffinity labeling of the ouabain-binding site on (Na+ plus K+) adenosinetriphosphatase.（钠钾）三磷酸腺苷酶上哇巴因结合位点的光亲和标记
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2352-6. doi: 10.1073/pnas.71.6.2352.
7
2-diazo-3,3,3-trifluoropropionyl chloride: reagent for photoaffinity labeling.2-重氮-3,3,3-三氟丙酰氯：光亲和标记试剂
Proc Natl Acad Sci U S A. 1976 May;73(5):1406-8. doi: 10.1073/pnas.73.5.1406.
8
Photoaffinity labeling of uncoupler binding sites on mitochondrial membrane.线粒体膜上解偶联剂结合位点的光亲和标记
J Bioenerg Biomembr. 1977 Feb;9(1):1-15. doi: 10.1007/BF00745039.
9
Fluorescent photoaffinity labeling: adenosine 3',5'-cyclic monophosphate receptor sites.荧光光亲和标记：3',5'-环磷酸腺苷受体位点
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1199-203. doi: 10.1073/pnas.75.3.1199.
本文引用的文献
1
An improved method of counting radioactive acrylamide gels.一种改进的放射性丙烯酰胺凝胶计数方法。
Anal Biochem. 1971 Aug;42(2):481-6. doi: 10.1016/0003-2697(71)90062-5.
2
Synthesis and characterization of photoaffinity labels for adenosine 3':5'-cyclic monophosphate and adenosine 5'-monophosphate.3':5'-环磷酸腺苷和5'-单磷酸腺苷光亲和标记物的合成与表征
Biochemistry. 1973 Oct 9;12(21):4074-8. doi: 10.1021/bi00745a008.
3
Surface changes in transformed cells detected by lectins.通过凝集素检测转化细胞的表面变化。
Fed Proc. 1973 Jan;32(1):91-101.
4
5
Photo-affinity labels for adenosine 3':5'-cyclic monophosphate.3':5'-环磷酸腺苷的光亲和标记物。
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1801-4. doi: 10.1073/pnas.68.8.1801.
6
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.人红细胞膜主要多肽的电泳分析。
Biochemistry. 1971 Jun 22;10(13):2606-17. doi: 10.1021/bi00789a030.
7
Induction of stalk cell differentiation by cyclic AMP in the cellular slime mold Dictyostelium discoideum.环磷酸腺苷诱导细胞黏菌盘基网柄菌中柄细胞分化
Proc Natl Acad Sci U S A. 1970 Jan;65(1):110-3. doi: 10.1073/pnas.65.1.110.
8
Relationships between erythrocyte membrane phosphorylation and adenosine triphosphate hydrolysis.红细胞膜磷酸化与三磷酸腺苷水解之间的关系。
J Biol Chem. 1968 Apr 25;243(8):1957-65.
Zotero 插件