Gauntt C J
Infect Immun. 1973 May;7(5):711-7. doi: 10.1128/iai.7.5.711-717.1973.
L cell cultures challenged with reoviruses types 1 and 3 produced little to no detectable interferon under conditions which permitted virus replication and under conditions which prevented replication of a temperature-sensitive mutant strain of reovirus type 3 (ts-1). Ultraviolet-irradiated reoviruses and double-stranded ribonucleic acids extracted from purified reovirus type 3 also induced little to no interferon in L cell cultures. Under similar conditions, MM virus proved to be an effective inducer of interferon. Exposure of L cells to interferon prior to challenge with virus (priming) failed to enhance interferon production upon subsequent challenge with reovirus although priming increased the amount of interferon produced following MM virus challenge. L cell cultures that were challenged with reovirus type 3 and subsequently with either MM or Colorado tick fever viruses produced similar titers of interferon as cell cultures that were challenged with either MM or Colorado tick fever virus alone, respectively. These data show that the presence of production of reovirus type 3 double-stranded ribonucleic acid is not sufficient for induction of interferon in L cell cultures and that additional processes which are required for induction of interferon in L cell cultures are not expressed by reovirus type 3.
在允许呼肠孤病毒1型和3型复制的条件下，以及在阻止呼肠孤病毒3型温度敏感突变株（ts-1）复制的条件下，用这两种病毒攻击L细胞培养物时，几乎检测不到或根本检测不到干扰素。紫外线照射的呼肠孤病毒以及从纯化的呼肠孤病毒3型中提取的双链核糖核酸，在L细胞培养物中也几乎不诱导或根本不诱导干扰素。在类似条件下，MM病毒被证明是一种有效的干扰素诱导剂。在用病毒攻击之前让L细胞接触干扰素（预刺激），在随后用呼肠孤病毒攻击时并不能增强干扰素的产生，尽管预刺激会增加在MM病毒攻击后产生的干扰素量。先用呼肠孤病毒3型攻击，随后再用MM病毒或科罗拉多蜱传热病毒攻击的L细胞培养物，产生的干扰素滴度与分别只用MM病毒或科罗拉多蜱传热病毒攻击的细胞培养物相似。这些数据表明，呼肠孤病毒3型双链核糖核酸的产生不足以在L细胞培养物中诱导干扰素，并且呼肠孤病毒3型不表达在L细胞培养物中诱导干扰素所需的其他过程。
