采用快速竞争性蛋白结合技术测定正常比格犬和灵缇犬外周血中皮质类固醇的水平。
Peripheral plasma levels of corticosteroids in normal beagles and greyhounds measured by a rapid competitive protein binding technique.
作者信息
Richkind M, Edqvist L E
出版信息
Acta Vet Scand. 1973 May;14(5):745-57. doi: 10.1186/BF03547402.
A rapid competitive protein binding method for the measurement of peripheral plasma levels of corticosteroids in dogs was evaluated and applied. The rapid method was found to give a minor overestimation of the Cortisol levels as compared to the method including purification of the plasma extract on Sephadex LH-20 columns. In Beagles and Greyhounds mean peripheral plasma levels of corticosteroids of 25.4 and 18.0 ng/ml were found respectively. No diurnal variation of peripheral plasma levels of corticosteroids was obtained. After the intravenous administration of dexamethasone peripheral plasma levels of corticosteroids were found to decrease to about 20 % of the pretreatment levels. When ACTH was administered i.m. a nearly 10 fold increase of the corticosteroid levels was found 60 to 90 min. after the injections. The rapid method described and evaluated should be of value for diagnosis of adrenal syndrome in the dog.
一种用于测量犬外周血浆中皮质类固醇水平的快速竞争性蛋白质结合方法得到了评估和应用。结果发现，与包括在Sephadex LH - 20柱上纯化血浆提取物的方法相比，该快速方法对皮质醇水平的估计略有高估。在比格犬和灵缇犬中，分别测得外周血浆皮质类固醇的平均水平为25.4和18.0 ng/ml。未观察到外周血浆皮质类固醇水平的昼夜变化。静脉注射地塞米松后，发现外周血浆皮质类固醇水平降至预处理水平的约20%。肌肉注射促肾上腺皮质激素（ACTH）后，注射后60至90分钟发现皮质类固醇水平增加了近10倍。所描述和评估的快速方法对于犬肾上腺综合征的诊断应该具有价值。
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