有证据表明，鸡肌腱原胶原蛋白必须变性才能作为脯氨酸羟化酶的底物。
Evidence that chick tendon procollagen must be denatured to serve as substrate for proline hydroxylase.
作者信息
Murphy L, Rosenbloom J
出版信息
Biochem J. 1973 Sep;135(1):249-51. doi: 10.1042/bj1350249.
Abstract
The ability of chick-embryo proline hydroxylase to hydroxylate [(14)C]proline-labelled procollagen was investigated between 23 degrees and 37 degrees C. The amount of hydroxy[(14)C]proline that could be formed increased sharply between 26 degrees and 30 degrees C. This corresponded to the temperature interval in which the [(14)C]procollagen substrate was thermally denatured, and the results therefore indicate that only denatured molecules can be hydroxylated.
摘要
在23摄氏度至37摄氏度之间研究了鸡胚脯氨酸羟化酶对[¹⁴C]脯氨酸标记的前胶原进行羟化的能力。在26摄氏度至30摄氏度之间，能够形成的羟[¹⁴C]脯氨酸的量急剧增加。这与[¹⁴C]前胶原底物发生热变性的温度区间相对应，因此结果表明只有变性的分子才能被羟化。
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