在连续未稀释传代过程中猴病毒40 DNA的重配
Reassortment of simian virus 40 DNA during serial undiluted passage.
作者信息
Martin M A, Gelb L D, Fareed G C, Milstien J B
出版信息
J Virol. 1973 Oct;12(4):748-57. doi: 10.1128/JVI.12.4.748-757.1973.
Abstract
Alterations occur in the supercoiled form of viral DNA after the serial undiluted passaging of simian virus (SV) 40. We have identified a portion of the viral genome which is amplified during this process. These SV40 DNA sequences represent about 30% of the viral genetic information and are present in a reiterated form in twisted circular molecules prepared from purified virions. In addition, reiterated and unique green monkey DNA sequences are incorporated into supercoiled viral DNA. The cellular DNA appears to be inserted at numerous locations in the DNA I molecules.
摘要
猿猴病毒（SV）40连续未稀释传代后，病毒DNA的超螺旋形式会发生改变。我们已经鉴定出病毒基因组的一部分，在这个过程中会被扩增。这些SV40 DNA序列约占病毒遗传信息的30%，以重复形式存在于从纯化病毒粒子制备的扭曲环状分子中。此外，重复的和独特的绿猴DNA序列被整合到超螺旋病毒DNA中。细胞DNA似乎插入到DNA I分子的多个位置。
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