Frenkeĺ N, Locker H, Batterson W, Hayward G S, Roizman B
J Virol. 1976 Nov;20(2):527-31. doi: 10.1128/JVI.20.2.527-531.1976.
We previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV-1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV-1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with 32P-labeled HSV-1 (Justin) defective DNA. The data allow us to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild-type viral DNA molecule.
我们之前报道过，单纯疱疹病毒1型（HSV-1）贾斯汀株（HSV-1 (Justin)）的连续传代导致产生了由有限复杂性序列的串联重复组成的缺陷性DNA分子。在本研究中，HSV-1 DNA用限制性内切酶BglII和EcoRI进行切割。片段在琼脂糖凝胶上进行电泳分离，转移到硝酸纤维素条上，然后与32P标记的HSV-1 (Justin)缺陷性DNA杂交。这些数据使我们能够得出结论，缺陷性DNA重复单元中包含的DNA序列源自野生型病毒DNA分子的S片段。
