Howley P M, Mullarkey M F, Takemoto K K, Martin M A
J Virol. 1975 Jan;15(1):173-81. doi: 10.1128/JVI.15.1.173-181.1975.
The DNA of the BK virus (BKV) human papovavirus was found to be heterogeneous, consisting of at least four discrete species of DNA. Only the largest of these four species, BKV DNA (i), which has a molecular weight calculated to be 96% that of simian virus 40 (SV40) DNA, was infectious. Homogeneous preparations of BKV DNA were obtained, however, from virions purified after low multiplicity infections of human embryonic kidney cells. BKV DNA (i) was shown to contain a single R-Eco RI and four R-Hind cleavage sites. The R-Eco RI site was localized in the largest R-Hind cleavage fragment. Radiolabeled BKV DNA reassociated slightly faster than SV40 DNA; 20 to 30% polynucleotide sequence homology was demonstrated between the genomes of BKV and SV40 when the reaction was monitored by chromatography on hydroxyapatite.
BK病毒（BKV）这种人乳头多瘤病毒的DNA被发现具有异质性，由至少四种不同的DNA种类组成。这四种种类中只有最大的一种，即BKV DNA（i）具有感染性，其计算分子量为猿猴病毒40（SV40）DNA的96%。然而，通过对人胚肾细胞进行低复数感染后纯化的病毒粒子，可获得BKV DNA的均一制剂。已表明BKV DNA（i）含有一个Eco RI酶切位点和四个Hind酶切位点。Eco RI酶切位点位于最大的Hind酶切片段中。放射性标记的BKV DNA重新结合的速度略快于SV40 DNA；当通过羟基磷灰石柱层析监测反应时，BKV和SV40的基因组之间显示出20%至30%的多核苷酸序列同源性。
