Wood H N, Rennekamp M E, Bowen D V, Field F H, Braun A C
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4140-3. doi: 10.1073/pnas.71.10.4140.
Two cell-division-promoting factors, which have partition coefficients of 1.9 and 2.75 determined by 500-tube countercurrent distribution in a butanolwater system, have been repeatedly isolated in this laboratory from crown gall tumor tissues of Vinca rosea L. These substances have been given the trivial names cytokinesin I and cytokinesin II, respectively. Chemical and mass spectrometric analyses suggest that both cytokinesins are substituted hypoxanthines and are thus very different compounds from the 6-substituted adenyl cytokinins. Carlos Miller, using a very different and far more drastic isolation procedure, obtained one main cell-division-promoting factor from these same tumor tissues, which he identified as ribosyl-trans-zeatin. On the basis of this finding, and without an attempt to repeat our studies, questions have been raised by Miller concerning the existence of the cytokinesins as biologically active substances. We have, therefore, compared some pertinent physical, chemical, and biological properties of the cytokinesins with those of zeatin riboside, have demonstrated that these three substances can be cleanly separated from one another by a number of different methods and that each behaves as a pure substance in the several systems, and, finally, we have shown that the cytokinesins are not contaminated with ribosyl-trans-zeatin and thus do not owe their biological activity to such a contaminant.
在本实验室中，通过在丁醇 - 水系统中进行500管逆流分配测定，发现长春花冠瘿瘤组织中有两种促进细胞分裂的因子，其分配系数分别为1.9和2.75。这两种物质分别被赋予了俗名细胞分裂素I和细胞分裂素II。化学分析和质谱分析表明，这两种细胞分裂素都是取代的次黄嘌呤，因此与6 - 取代的腺苷细胞分裂素是非常不同的化合物。卡洛斯·米勒采用了一种截然不同且更为剧烈的分离方法，从这些相同的肿瘤组织中获得了一种主要的促进细胞分裂的因子，他将其鉴定为核糖基 - 反式玉米素。基于这一发现，且未尝试重复我们的研究，米勒对细胞分裂素作为生物活性物质的存在提出了质疑。因此，我们比较了细胞分裂素与玉米素核苷的一些相关物理、化学和生物学性质，证明这三种物质可以通过多种不同方法清晰地彼此分离，并且在几个系统中每种物质都表现为纯物质，最后，我们表明细胞分裂素未被核糖基 - 反式玉米素污染，因此其生物活性并非归因于这种污染物。
