Martin H G, Thorne K J
Biochem J. 1974 Feb;138(2):281-9. doi: 10.1042/bj1380281.
Endogenous dolichol was shown to function as a natural acceptor of mannose residues by using regenerating rat liver containing [(3)H]dolichol. When subcellular fractions from this liver were incubated with GDP-[(14)C]mannose a double-labelled lipid, which represented 30% of the total [(14)C]mannolipid, could be isolated. This lipid was shown to be identical with the dolichol phosphate mannose formed from exogenous dolichol phosphate, by chromatography, stability to alkali and by chemical cleavage to mannose and dolichol derivatives. It was formed by the rough endoplasmic reticulum and mitochondria. If it is concerned in glycoprotein synthesis this would suggest that it functions in the formation of both secreted and mitochondrial glycoproteins. When both the dolichol and retinol of rat tissue were radioactive they made similar contributions to the synthesis of the lipid by liver microsomal fractions and intestinal epithelial cells.
通过使用含有[(3)H]多萜醇的再生大鼠肝脏,内源性多萜醇被证明可作为甘露糖残基的天然受体。当将该肝脏的亚细胞组分与GDP-[(14)C]甘露糖一起孵育时,可以分离出一种双标记脂质,其占总[(14)C]甘露糖脂的30%。通过色谱法、对碱的稳定性以及化学裂解为甘露糖和多萜醇衍生物,表明该脂质与由外源性磷酸多萜醇形成的磷酸多萜醇甘露糖相同。它是由糙面内质网和线粒体形成的。如果它参与糖蛋白合成,这将表明它在分泌型和线粒体糖蛋白的形成中都起作用。当大鼠组织的多萜醇和视黄醇都具有放射性时,它们对肝脏微粒体组分和肠上皮细胞合成脂质的贡献相似。