在再生大鼠肝脏中由（4S-3H）甲羟戊酸合成放射性多萜醇。
Synthesis of radioactive dolichol from (4S-3H)mevalonate in the regenerating rat liver.
作者信息
Martin H G, Thorne K J
出版信息
Biochem J. 1974 Feb;138(2):277-80. doi: 10.1042/bj1380277.
Abstract
The dolichol of rat liver was labelled by injecting [4S-(3)H]mevalonate, the precursor of cis-isoprene residues, into partially hepatectomized animals. The optimum conditions for labelling the dolichol were to inject the animals with radioactive mevalonate 48h after hepatectomy and to kill them 12h later. The concentration of radioactive dolichol was five times as great in regenerating rat liver as in normal liver. The highest concentration of radioactive dolichol was found in the crude mitochondrial and nuclear-debris fractions of the cell. The crude microsomal fractions also contained radioactive dolichol, but at a lower concentration.
摘要
通过向部分肝切除的动物注射顺式异戊二烯残基的前体[4S-(3)H]甲羟戊酸，对大鼠肝脏的多萜醇进行标记。标记多萜醇的最佳条件是在肝切除后48小时给动物注射放射性甲羟戊酸，并在12小时后将其处死。再生大鼠肝脏中放射性多萜醇的浓度是正常肝脏中的五倍。在细胞的粗线粒体和核碎片部分发现放射性多萜醇的浓度最高。粗微粒体部分也含有放射性多萜醇，但浓度较低。
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