喜树碱的抗病毒作用。
Antiviral action of camptothecin.
作者信息
Horwitz S B, Chang C K, Grollman A P
出版信息
Antimicrob Agents Chemother. 1972 Nov;2(5):395-401. doi: 10.1128/AAC.2.5.395.
Abstract
At a concentration of 10 mum, camptothecin inhibited vaccinia deoxyribonucleic acid (DNA) synthesis in HeLa cells. Inhibition of viral DNA synthesis was observed when the drug was added before infection or at 1 or 2 hr after infection. Inhibitory effects of camptothecin on vaccinia DNA synthesis could be reversed, even after exposure to the alkaloid for 2 hr. Viral DNA, isolated from vaccinia-infected, camptothecin-treated cells, displayed an altered sedimentation constant after alkaline sucrose density gradient centrifugation. Incorporation of uridine into vaccinia messenger ribonucleic acid was inhibited by camptothecin, but the activity of ribonucleic acid polymerase, as tested in isolated vaccinia cores, was not affected by the drug. Camptothecin had essentially no effect on replication of poliovirus in HeLa cells.
摘要
在浓度为10微摩尔时，喜树碱抑制了HeLa细胞中牛痘病毒脱氧核糖核酸（DNA）的合成。当在感染前或感染后1或2小时添加该药物时，可观察到病毒DNA合成受到抑制。即使在接触该生物碱2小时后，喜树碱对牛痘病毒DNA合成的抑制作用仍可逆转。从感染牛痘病毒且经喜树碱处理的细胞中分离出的病毒DNA，在碱性蔗糖密度梯度离心后沉降常数发生了改变。喜树碱抑制了尿苷掺入牛痘病毒信使核糖核酸，但在分离的牛痘病毒核心中检测的核糖核酸聚合酶活性不受该药物影响。喜树碱对脊髓灰质炎病毒在HeLa细胞中的复制基本没有影响。
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