Bolender R P
J Cell Biol. 1974 May;61(2):269-87. doi: 10.1083/jcb.61.2.269.
A stereological model which provides detailed quantitative information on the structure of the fasted, nonstimulated gland has been developed for the guinea pig pancreas. The model consists of morphologically defined space and membrane compartments which were used to describe the general composition of the tissue and the specific components of exocrine cells. The results are presented, where appropriate, relative to a cubic centimeter of pancreas, a cubic centimeter of exocrine cell cytoplasm, and to the volume of an average exocrine cell. The exocrine cells, accounting for 82% of the pancreas volume, consisted of 54% cytoplasmic matrix, 22% rough-surfaced endoplasmic reticulum (RER), 8.3% nuclei, 8.1% mitochondria, 6.4% zymogen granules, and 0.7% condensing vacuoles. Their total membrane surface area was distributed as follows: 60% RER, 21% mitochondria, 9.9% Golgi apparatus, 4.8% plasma membranes, 2.6% zymogen granules, 1.8% plasma membrane vesicles, and 0.4% condensing vacuoles. The application of this model to the study of membrane movements associated with the secretory process is discussed within the framework of an analytical approach.
已为豚鼠胰腺建立了一个立体学模型，该模型可提供禁食、未受刺激腺体结构的详细定量信息。该模型由形态学定义的空间和膜隔室组成，用于描述组织的总体组成和外分泌细胞的特定成分。在适当情况下，结果以相对于一立方厘米胰腺、一立方厘米外分泌细胞质以及一个平均外分泌细胞的体积来呈现。外分泌细胞占胰腺体积的82%，由54%的细胞质基质、22%的糙面内质网（RER）、8.3%的细胞核、8.1%的线粒体、6.4%的酶原颗粒和0.7%的浓缩泡组成。它们的总膜表面积分布如下：60%为RER、21%为线粒体、9.9%为高尔基体、4.8%为质膜、2.6%为酶原颗粒、1.8%为质膜小泡、0.4%为浓缩泡。在分析方法的框架内讨论了该模型在与分泌过程相关的膜运动研究中的应用。
