用于估算6-氨基青霉烷酸的分光光度法。
Spectrophotometric method for the estimation of 6-aminopenicillanic acid.
作者信息
Shaikh K, Talati P G, Gang D M
出版信息
Antimicrob Agents Chemother. 1973 Feb;3(2):194-7. doi: 10.1128/AAC.3.2.194.
Abstract
A simple, rapid, and sensitive method is described whereby 6-aminopenicillanic acid can be spectrophotometrically determined in the presence of penicillins and their degradation products without prior separation. d-(+)-Glucosamine is used as reagent. The effect of such parameters as pH, temperature, and time of heating on the formation of the chromophore is described. The recommended range is from 25 to 250 mug of 6-aminopenicillanic acid.
摘要
本文描述了一种简单、快速且灵敏的方法，无需预先分离，即可在青霉素及其降解产物存在的情况下，通过分光光度法测定6-氨基青霉烷酸。使用d-(+)-葡萄糖胺作为试剂。描述了诸如pH值、温度和加热时间等参数对发色团形成的影响。推荐的6-氨基青霉烷酸测定范围为25至250微克。
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AUTOMATED COLORIMETRIC DETERMINATION OF 6-AMINOPENICILLANIC ACID IN FERMENTATION MEDIA.发酵培养基中6-氨基青霉烷酸的自动比色测定法
Anal Chem. 1965 Apr;37:576-8. doi: 10.1021/ac60223a034.
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A MANOMETRIC METHOD FOR THE ESTIMATION OF PENICILLIN AMIDASE.一种测定青霉素酰胺酶的测压法。
Indian J Biochem. 1964 Dec;1:224-6.
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Action of carbon dioxide on 6-aminopencillanic acid.二氧化碳对6-氨基青霉烷酸的作用。
Nature. 1961 Aug 26;191:910-1. doi: 10.1038/191910a0.
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