在能够进行核糖核酸和蛋白质合成的大肠杆菌透化细胞中的核糖核酸调控
Ribonucleic acid regulation in premeabilized cells of Escherichia coli capable of ribonucleic acid and protein synthesis.
作者信息
Atherly A G
出版信息
J Bacteriol. 1974 Jun;118(3):1186-9. doi: 10.1128/jb.118.3.1186-1189.1974.
Abstract
A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis.
摘要
本文描述了一种细胞通透化方法，该方法使细胞活力降低不到10%，并且在适当补充时不会显著降低核糖核酸和蛋白质的合成速率。通透化消除了蛋白质和核糖核酸合成之间正常的严格偶联。
相似文献
1
Ribonucleic acid regulation in premeabilized cells of Escherichia coli capable of ribonucleic acid and protein synthesis.
J Bacteriol. 1974 Jun;118(3):1186-9. doi: 10.1128/jb.118.3.1186-1189.1974.
2
Regulation of RNA synthesis in Escherichia coli. I. Characterization of cells subjected to simultaneous temperature and osmotic shock.
Biochim Biophys Acta. 1973 Jul 27;312(4):722-36. doi: 10.1016/0005-2787(73)90076-2.
3
Temperature-sensitive mutation in regulation of ribonucleic acid synthesis in Escherichia coli.
J Bacteriol. 1972 Dec;112(3):1150-6. doi: 10.1128/jb.112.3.1150-1156.1972.
4
The control of ribonucleic acid synthesis in bacteria. The synthesis and stbility of ribonucleic acid in rifampicin-inhibited cultures of Escherichia coli.
Biochem J. 1971 Apr;122(2):161-9. doi: 10.1042/bj1220161.
5
Ribonucleic acid regulation in amino acid-limited cultures of Escherichia coli grown in a chemostat.
J Bacteriol. 1974 Dec;120(3):1322-30. doi: 10.1128/jb.120.3.1322-1330.1974.
6
Decay of ribonucleic acid synthesis in amino acid-starved Escherichia coli after rifampin treatment.
J Bacteriol. 1974 Mar;117(3):987-93. doi: 10.1128/jb.117.3.987-993.1974.
7
Stimulation of RNA synthesis at initiation by ribosomal proteins.
Arch Biochem Biophys. 1974 Apr 2;161(2):705-8. doi: 10.1016/0003-9861(74)90358-0.
8
Characterization of mutants of Escherichia coli temperature-sensitive for ribonucleic acid regulation: an unusual phenotype associated with a phenylalanyl transfer ribonucleic acid synthetase mutant.
J Bacteriol. 1971 Nov;108(2):627-38. doi: 10.1128/jb.108.2.627-638.1971.
9
Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. 1. Ribosomal RNA chain growth rates.
J Mol Biol. 1973 Mar 25;75(1):145-59. doi: 10.1016/0022-2836(73)90535-4.
10
Studies on the in vitro transcription and translation of the lac operon.
Arch Biochem Biophys. 1974 Jan;160(1):168-74. doi: 10.1016/s0003-9861(74)80023-8.
引用本文的文献
1
Immobilization of Escherichia coli RNA polymerase and location of binding sites by use of chromatin immunoprecipitation and microarrays.
J Bacteriol. 2005 Sep;187(17):6166-74. doi: 10.1128/JB.187.17.6166-6174.2005.
2
Deoxyribonucleic acid replication in permeable and fully viable Escherichia coli cells.
J Bacteriol. 1981 Mar;145(3):1413-6. doi: 10.1128/jb.145.3.1413-1416.1981.
3
Characterization of RNA synthesis in an Escherichia coli mutant with a temperature-sensitive lesion in stable RNA synthesis.
J Bacteriol. 1983 Feb;153(2):616-26. doi: 10.1128/jb.153.2.616-626.1983.
4
Transcription of exogenous and endogenous deoxyribonucleic acid templates in cold-shocked Bacillus subtilis.
J Bacteriol. 1980 Sep;143(3):1345-52. doi: 10.1128/jb.143.3.1345-1352.1980.
5
Effects of guanosine tetraphosphate on cell-free synthesis of Escherichia coli ribosomal RNA and other gene products.
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2881-5. doi: 10.1073/pnas.72.8.2881.
6
Preferential ribosomal RNA synthesis in the lysate of Escherichia coli.
Mol Gen Genet. 1975;138(1):1-10. doi: 10.1007/BF00268822.
7
Stability of Escherichia coli membrane proteins during chloramphenicol treatment.
Antimicrob Agents Chemother. 1975 Sep;8(3):387-9. doi: 10.1128/AAC.8.3.387.
8
Simple downshift and resulting lack of correlation between ppGpp pool size and ribonucleic acid accumulation.
J Bacteriol. 1975 May;122(2):585-91. doi: 10.1128/jb.122.2.585-591.1975.
9
Regulation of ribonucleic acid synthesis in spheroplasts, cold-shocked cells, and toluene-treated cells of Escherichia coli.
J Bacteriol. 1976 Oct;128(1):123-9. doi: 10.1128/jb.128.1.123-129.1976.
10
Selective methylation: an incorrect hypothesis.
J Bacteriol. 1976 May;126(2):1009-11. doi: 10.1128/jb.126.2.1009-1011.1976.
本文引用的文献
1
Regulation of ribosomal RNA synthesis in cold-shocked E. coli.
Nat New Biol. 1973 May 2;243(122):17-20.
2
ENZYME INDUCTION AS AN ALL-OR-NONE PHENOMENON.
Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):553-66. doi: 10.1073/pnas.43.7.553.
3
ACTINOMYCIN SENSITIVITY IN ESCHERICHIA COLI PRODUCED BY EDTA.
Biochem Biophys Res Commun. 1965 Jan 4;18:13-7. doi: 10.1016/0006-291x(65)90874-0.
4
Effects of toluene on Escherichia coli.
J Bacteriol. 1965 Nov;90(5):1420-5. doi: 10.1128/jb.90.5.1420-1425.1965.
5
Effect of ethylenediaminetetraacetic acid-Tris(hydroxymethyl)aminomethane on release of the acid-soluble nucleotide pool and on breakdown of ribosomal ribonucleic acid in Escherichia coli.
J Bacteriol. 1967 Apr;93(4):1360-8. doi: 10.1128/jb.93.4.1360-1368.1967.
6
Selective release of ribonuclease-inhibitor from Bacillus subtilis cells by cold shock treatment.
Biochem Biophys Res Commun. 1967 Jan 10;26(1):75-81. doi: 10.1016/0006-291x(67)90255-0.
7
Characterization of mutants of Escherichia coli temperature-sensitive for ribonucleic acid regulation: an unusual phenotype associated with a phenylalanyl transfer ribonucleic acid synthetase mutant.
J Bacteriol. 1971 Nov;108(2):627-38. doi: 10.1128/jb.108.2.627-638.1971.
8
Titration of the gene sites on DNA by DNA-RNA hybridization. II. The Escherichia coli chromosome.
J Mol Biol. 1968 May 28;34(1):85-103. doi: 10.1016/0022-2836(68)90236-2.
Zotero 插件