在能够进行核糖核酸和蛋白质合成的大肠杆菌透化细胞中的核糖核酸调控
Ribonucleic acid regulation in premeabilized cells of Escherichia coli capable of ribonucleic acid and protein synthesis.
作者信息
Atherly A G
出版信息
J Bacteriol. 1974 Jun;118(3):1186-9. doi: 10.1128/jb.118.3.1186-1189.1974.
Abstract
A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis.
摘要
本文描述了一种细胞通透化方法，该方法使细胞活力降低不到10%，并且在适当补充时不会显著降低核糖核酸和蛋白质的合成速率。通透化消除了蛋白质和核糖核酸合成之间正常的严格偶联。
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本文引用的文献
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Regulation of ribosomal RNA synthesis in cold-shocked E. coli.冷休克大肠杆菌中核糖体RNA合成的调控
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Effect of ethylenediaminetetraacetic acid-Tris(hydroxymethyl)aminomethane on release of the acid-soluble nucleotide pool and on breakdown of ribosomal ribonucleic acid in Escherichia coli.乙二胺四乙酸-三（羟甲基）氨基甲烷对大肠杆菌中酸溶性核苷酸库释放及核糖体核糖核酸降解的影响。
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Selective release of ribonuclease-inhibitor from Bacillus subtilis cells by cold shock treatment.通过冷休克处理从枯草芽孢杆菌细胞中选择性释放核糖核酸酶抑制剂。
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Characterization of mutants of Escherichia coli temperature-sensitive for ribonucleic acid regulation: an unusual phenotype associated with a phenylalanyl transfer ribonucleic acid synthetase mutant.对核糖核酸调节温度敏感的大肠杆菌突变体的特性研究：与苯丙氨酰转移核糖核酸合成酶突变体相关的一种异常表型。
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8
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